公开(公告)号：US3915803A

公开(公告)日：1975-10-28

申请号：US49831174

申请日：1974-08-19

申请人： UPJOHN CO

发明人： KOMINEK LEO A

IPC分类号： C12P17/16 ,  C07D307/73 ,  C12P1/06 ,  C12P17/12 ,  C12R1/465 ,  C12D9/00

CPC分类号： C07D307/73 ,  Y10S435/813 ,  Y10S435/897

摘要： It has been found that cycloheximide production by a culture of Streptomyces griseus is maintained and total yields of the antibiotic are increased when a glucose feed is begun at about 48 hrs. and continued until the culture becomes senescent at about 8 to 10 days.

摘要翻译： 已经发现，当葡萄糖进料在约48小时开始时，通过维持链格孢菌链霉菌培养物的放线菌酮生产得以维持并且总产量增加。 并持续至文化在约8至10天变得衰老。

公开(公告)号：US3915802A

公开(公告)日：1975-10-28

申请号：US49831274

申请日：1974-08-19

申请人： UPJOHN CO

发明人： KOMINEK LEO A

IPC分类号： C12P17/12 ,  B01D61/28 ,  C12M1/02 ,  C12M1/12 ,  C12N1/00 ,  C12P1/00 ,  C12B1/00

CPC分类号： B01D61/28 ,  B01D63/06 ,  B01D71/12 ,  C12M29/04 ,  C12M47/10 ,  Y10S435/803 ,  Y10S435/897

摘要： A new dialysis method and apparatus is described for separating, accumulating, and recovering cycloheximide from a culture medium wherein Streptomyces griseus is producing the antibiotic. The new method and apparatus utilizes an aqueous pick-up medium (a diffusate) which is flowed through tubular structure of a semipermeable membrane means through which membrane cycloheximide will diffuse from a fermentation culture medium producing the same. The flowing diffusate, having accumulated cycloheximide in its passage through the membrane structure, is discharged into a water-immiscible organic solvent where the cycloheximide is extracted from the diffusate because of its greater solubility in the organic solvent. The extracted aqueous medium collects in an aqueous phase separate from the organic solvent phase. The aqueous phase is drawn off (preferably stripped of any dissolved organic solvent) and returned to the semi-permeable membrane means for further separation and accumulation of diffusing cycloheximide and discharge into the extracting solvent. The same procedure is applicable to other biochemicals produced by microbial cultures.

摘要翻译： 描述了一种新的透析方法和装置，用于从灰霉链霉菌（Streptomyces griseus）生产抗生素的培养基中分离，积聚和回收放线菌酮。 新的方法和装置利用透过膜的方法流过管状结构的水性吸收介质（扩散剂），通过该半透膜的装置，放线菌酮膜将从产生其的发酵培养基中扩散。 在其通过膜结构的过程中积累的放线菌酮的流动扩散剂被排放到与水不混溶的有机溶剂中，其中放线菌酮从扩散物中提取，因为其在有机溶剂中的溶解度更高。 萃取的水性介质在与有机溶剂相分离的水相中收集。 将水相抽出（优选剥离任何溶解的有机溶剂）并返回到半透膜装置，用于进一步分离和积累扩散的放线菌酮并排放到萃取溶剂中。 相同的程序适用于微生物培养生产的其他生物化学品。