公开(公告)号：US11242552B2

公开(公告)日：2022-02-08

申请号：US17044734

申请日：2019-02-01

Applicant: USTAR Biotechnologies (Hangzhou) Ltd.

Inventor： Qimin You ,  Lin Hu ,  Chen Qi ,  Junwei Yu ,  Zhujun Yu ,  Sha Wang ,  Rongyu Jin ,  Daisang Wang ,  Sisi Chen ,  Junli He ,  Jing Chen ,  Huanxin Rao ,  Yanqiong Zhou ,  Fan Yang

IPC: C12Q1/68 ,  C12Q1/6806 ,  B01J19/00

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.