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公开(公告)号：US20250129410A1
公开(公告)日：2025-04-24
申请号：US18837232
申请日：2023-02-27
IPC: C12Q1/682
Abstract: Described herein are methods, compositions, and kits relating to the mixed detection of one or more polynucleotides, in particular miRNAs. In certain aspects, methods, systems, compositions, and kits utilize Cas12a for polynucleotide detection, in particular miRNAs. In aspects the methods, systems, compositions, and kits utilize Cas12a for detection of polynucleotide targets in a single pot reaction.
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