公开(公告)号：US20210308680A1

公开(公告)日：2021-10-07

申请号：US17266217

申请日：2019-09-24

Applicant: University of Manitoba

Inventor： Francis Lin ,  Jiandong Wu ,  Claudio Rigatto ,  Paul Komenda ,  Navdeep Tangri ,  Gong Zhang

IPC: B01L3/00 ,  G01N33/68 ,  G01N33/70 ,  G01N21/78

Abstract: Described herein is a method for mixing unequal amounts of two reagents to produce a detectable reaction in a microfluidic chip. In one example, there is a fluorescent microfluidic urinary albumin chip (UAL-Chip) that exploits the nonimmunological fluorescent assay. In this chip, we constructed a passive and continuous mixing module, in which the loading process requires only an inexpensive dropper, and the signal is stable over time, as discussed below. We applied a pressure-balancing strategy based on the immiscible oil coverage which highly improves the precision in controlling the mixing ratio of sample and dye. The UAL-Chip has achieved an estimated limit of detection (LOD) of 8.4 μg/ml using albumin standards, which is below the 30 μg albumin per ml urine level considered to be indicative of kidney damage.

公开(公告)号：US12186753B2

公开(公告)日：2025-01-07

申请号：US17266217

申请日：2019-09-24

Applicant: University of Manitoba

Inventor： Francis Lin ,  Jiandong Wu ,  Claudio Rigatto ,  Paul Komenda ,  Navdeep Tangri ,  Gong Zhang

IPC: B01L3/00 ,  G01N21/78 ,  G01N33/68 ,  G01N33/70

Abstract: Described herein is a method for mixing unequal amounts of two reagents to produce a detectable reaction in a microfluidic chip. In one example, there is a fluorescent microfluidic urinary albumin chip (UAL-Chip) that exploits the nonimmunological fluorescent assay. In this chip, we constructed a passive and continuous mixing module, in which the loading process requires only an inexpensive dropper, and the signal is stable over time, as discussed below. We applied a pressure-balancing strategy based on the immiscible oil coverage which highly improves the precision in controlling the mixing ratio of sample and dye. The UAL-Chip has achieved an estimated limit of detection (LOD) of 8.4 μg/ml using albumin standards, which is below the 30 μg albumin per ml urine level considered to be indicative of kidney damage.