公开(公告)号：US20220259574A1

公开(公告)日：2022-08-18

申请号：US17434351

申请日：2020-02-27

Applicant: University of Virginia Patent Foundation

Inventor： Huiwang Ai ,  Hsien-Wei Yeh ,  Ying Xiong

IPC: C12N9/02 ,  C12N15/63 ,  G01N33/542

Abstract: Provided herein are chemically modified luciferase substrates for spectrally shifted emission and enhanced water solubility. Provided herein are engineered luciferases. Moreover, provided herein are new ATP-independent bioluminescent reporters which have improved biochemical and photophysical properties and are expected to have broad applications. Finally, provided herein are spectral-resolved triple-color bioluminescent systems, suitable for flexible and convenient approaches to monitor multiple biological events in either qualitative or quantitative manners