公开(公告)号：US20040009529A1

公开(公告)日：2004-01-15

申请号：US10317853

申请日：2002-12-11

Applicant: Utah State University

Inventor： Bart C. Weimer ,  Marie K. Walsh

IPC: G01N033/53 ,  G01N033/543

CPC classification number: G01N33/54353 ,  G01N33/5304 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/56911

Abstract: Antigens can be captured and detected from complex samples, such as food and environmental samples, in about 30 minutes using apparatus and methods that include flow of the samples through a module containing antibodies coupled to beads. The samples flow through the modified beads at about 0.2 to 1.2 liters/minute, which fluidizes the bead bed. The antigens are captured by the antibodies, and then detection of the captured antibodies is carried out with chemiluminescence, fluorescence, or spectrophotometric techniques.

Abstract translation: 使用装置和方法可以在约30分钟内从复杂样品（例如食物和环境样品）中捕获和检测抗原，所述设备和方法包括通过包含偶联珠子的抗体的模块的样品流动。 样品以约0.2至1.2升/分钟流过改性珠粒，使珠粒床流化。 抗原被抗体捕获，然后用化学发光，荧光或分光光度技术检测捕获的抗体。