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公开(公告)号:US20050186588A1
公开(公告)日:2005-08-25
申请号:US10967711
申请日:2004-10-18
申请人: Victor Lyamichev , Andrew Lukowiak , Nancy Jarvis , David Kurensky , Hatim Allawi , Scott Law , Chad Gerber , Robert Roeven , Jeff Hall
发明人: Victor Lyamichev , Andrew Lukowiak , Nancy Jarvis , David Kurensky , Hatim Allawi , Scott Law , Chad Gerber , Robert Roeven , Jeff Hall
IPC分类号: C12P19/34 , C12Q20060101 , C12Q1/68
CPC分类号: C12Q1/6846 , C12Q2527/119 , C12Q2527/125 , C12Q2561/109
摘要: The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.
摘要翻译: 本发明提供用于开发和优化用于基础研究,临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。 特别地,本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。 本发明还提供优化多重扩增反应的方法。 本发明还提供了用于组合的靶和信号产生测定的方法。