公开(公告)号：US4407947A

公开(公告)日：1983-10-04

申请号：US302022

申请日：1981-09-14

申请人： Volker Koppenhagen ,  Gerhard Schlingmann ,  Bernd Dresow ,  Ludwig Bischoff ,  Rosemarie Penkert ,  Eicke Siefert

发明人： Volker Koppenhagen ,  Gerhard Schlingmann ,  Bernd Dresow ,  Ludwig Bischoff ,  Rosemarie Penkert ,  Eicke Siefert

IPC分类号： C12P17/18 ,  C12P19/42 ,  C12R1/01 ,  C12P19/28

CPC分类号： C12P19/42 ,  C12P17/182

摘要： A process for obtaining metal-free corrinoids consisting of inoculating a culture media free of added compounds of metals of the VIIIth group of the Periodic Table with a parent strain of Rhodopseudomonas, cultivating under illumination, repeating the steps of inoculating and cultivating employing said culture media and said illumination employing a part of the sequential culture from the preceding stage as an inoculum in each subsequent state, for a series of several pre-production culture stages, inoculating said culture media with the preceding sequential culture for the last time, cultivating under illumination, separating the microorganism from the culture media of the production culture stage and isolating metal-free corrinoids from the culture media of the production culture stage.

摘要翻译： 一种获得无金属类胡萝卜素的方法，该方法包括将不含周期表第八组的金属的添加化合物的培养基与红假单胞菌的亲本菌株接种，在照射下培养，重复使用所述培养基接种和培养的步骤 并将所述照射采用来自前一阶段的连续培养物的一部分作为每种后续状态的接种物，用于一系列预生产培养阶段，最后接种具有先前顺序培养的所述培养基，在照明条件下培养 从生产培养阶段的培养基中分离微生物，并从生产培养阶段的培养基中分离出无金属的类固醇。