Tissue processing for immunofluorescence microscopy
    1.
    发明授权
    Tissue processing for immunofluorescence microscopy 失效
    免疫荧光显微镜的组织加工

    公开(公告)号:US4971783A

    公开(公告)日:1990-11-20

    申请号:US443838

    申请日:1982-11-22

    IPC分类号: G01N1/30

    CPC分类号: G01N1/30 Y10S435/96

    摘要: A tissue holding and processing technique suitable for immunofluorescence, histochemical, and light microscopic analysis of the same specimen. The tissue is first placed in formol sucrose, and then transferred to gum sucrose. The tissue is subsequently washed in phosphate buffered saline, then acetone-water, then acetone, and finally in xylene. Finally, the tissue is embedded in paraffin. Tissue processed according to the present invention may be stored indefinitely in the gum sucrose or paraffin.

    摘要翻译: 一种适用于相同标本的免疫荧光,组织化学和光学显微镜分析的组织保持和加工技术。 将组织首先置于甲醛蔗糖中,然后转移至蔗糖。 随后将组织在磷酸盐缓冲盐水中洗涤,然后用丙酮 - 水,然后用丙酮洗涤,最后在二甲苯中洗涤。 最后,将组织包埋在石蜡中。 根据本发明加工的组织可以无限期地储存在蔗糖或石蜡中。