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1.发明授权cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells 有权反映人类多能干细胞生长和分化过程中基因表达的cDNA文库公开(公告)号：US06667176B1
公开(公告)日：2003-12-23
申请号：US09688031
申请日：2000-10-10
IPC分类号： C12N506
CPC分类号： C12N5/0606 , A61K35/12 , C12N15/1034 , C12N15/1072 , C12N15/1096 , C12N2500/14 , C12N2500/30 , C12N2500/38 , C12N2500/44 , C12N2500/62 , C12N2501/105 , C12N2501/13 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/999 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2510/00 , C12N2510/04 , C12N2533/90
摘要： This disclosure provides a system for obtaining expression libraries from primate pluripotent stem (pPS) cells. pPS cells can be maintained in vitro without requiring a layer of feeder cells to inhibit differentiation. The role of the feeder cells is replaced by several other culture conditions provided in a suitable combination. Conditions that promote pPS cell growth without differentiation include supporting the culture on an extracellular matrix, and culturing the cells in a medium conditioned by another cell type. The cDNA libraries from such cultures are devoid of transcripts of feeder cell origin, relatively uncontaminated by transcripts from differentiated cells, and can have a high proportion of full-length transcripts. Subtraction libraries can also be produced that are enriched for transcripts modulated during differentiation.
摘要翻译： 本公开提供了用于从灵长动物多能干（pPS）细胞获得表达文库的系统。 可以在体外维持pPS细胞，而不需要一层饲养细胞抑制分化。 饲养细胞的作用由适当组合提供的几种其它培养条件代替。 促进没有分化的pPS细胞生长的条件包括在细胞外基质上支持培养物，并在由另一细胞类型调节的培养基中培养细胞。 来自这些培养物的cDNA文库没有饲养细胞来源的转录物，相对来自分化细胞的转录物不受污染，并且可以具有高比例的全长转录物。 还可以产生减毒文库，其富集分化过程中调节的转录本。
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公开(公告)号：US06599728B2
公开(公告)日：2003-07-29
申请号：US09972115
申请日：2001-10-05
IPC分类号： C12N912
CPC分类号： C12Q1/6886 , C12Q2600/136
摘要： A new protein named Tankyrase II is described in this disclosure. Sequences for the human Tankyrase II cDNA and the protein translation product are provided. Also provided are species homologs, muteins, related nucleic acids, peptides, and drug screening assays. Tankyrase II interacts with telomere-associated proteins, thereby affecting telomerase activity and potentially telomere length. The materials and techniques provided in this disclosure allow Tankyrase II activity to be studied in vitro and manipulated inside cells—to the potential benefit of clinical conditions associated with a defect in telomerase activity, or the replicative capacity of affected cells.
摘要翻译： 在本公开中描述了名为Tankyrase II的新蛋白质。 提供人类Tankyrase II cDNA和蛋白质翻译产物的序列。 还提供了物种同系物，突变蛋白，相关核酸，肽和药物筛选测定。 端粒酶II与端粒相关蛋白相互作用，从而影响端粒酶活性和潜在的端粒长度。 在本公开中提供的材料和技术允许在体外研究槽状酶II活性并在细胞内操作 - 与端粒酶活性缺陷或受影响细胞的复制能力相关的临床病症的潜在益处。
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3.发明授权公开(公告)号：US08080640B2
公开(公告)日：2011-12-20
申请号：US10887711
申请日：2004-07-08
摘要： Recombinant transferrin, non-glycosylated recombinant transferrin, transferrin half-molecules and mutant transferrins having altered metal-binding or other properties are described. The recombinant transferrin molecules are expressed in functional form by stable eukaryotic cell lines such as baby hamster kidney cells transformed with an expression vector encoding the recombinant molecule. The recombinant transferrins can be used in metal chelation therapy to bind and clear excess toxic metals in patients suffering from metal overloads or as tissue culture medium supplements or replacements.
摘要翻译： 描述了具有改变的金属结合或其它性质的重组转铁蛋白，非糖基化重组转铁蛋白，转铁蛋白半分子和突变体转运蛋白。 重组转铁蛋白分子以稳定的真核细胞系如用编码重组分子的表达载体转化的幼仓鼠肾细胞以功能形式表达。 重组转移蛋白可用于金属螯合治疗，以结合和清除患有金属超负荷或组织培养基补充剂或替代物的患者中的过量有毒金属。
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4.发明授权公开(公告)号：US06825037B1
公开(公告)日：2004-11-30
申请号：US09439740
申请日：1999-11-15
IPC分类号： C12N510
摘要： Recombinant transferrin, non-glycosylated recombinant transferrin, transferrin half-molecules and mutant transferrins having altered metal-binding or other properties are described. The recombinant transferrin molecules are expressed in functional form by stable eukaryotic cell lines such as baby hamster kidney cells transformed with an expression vector encoding the recombinant molecule. The recombinant transferrins can be used in metal chelation therapy to bind and clear excess toxic metals in patients suffering from metal overloads or as tissue culture medium supplements or replacements.
摘要翻译： 描述了具有改变的金属结合或其它性质的重组转铁蛋白，非糖基化重组转铁蛋白，转铁蛋白半分子和突变体转运蛋白。 重组转铁蛋白分子以稳定的真核细胞系如用编码重组分子的表达载体转化的幼仓鼠肾细胞以功能形式表达。 重组转移蛋白可用于金属螯合治疗，以结合和清除患有金属超负荷或组织培养基补充剂或替代物的患者中的过量有毒金属。
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5.发明授权
公开(公告)号：US5986067A
公开(公告)日：1999-11-16
申请号：US175158
申请日：1993-12-28
摘要： Recombinant transferrin, non-glycosylated recombinant transferrin, transferrin half-molecules and mutant transferrins having altered metal-binding or other properties are described. The recombinant transferrin molecules are expressed in functional form by stable eukaryotic cell lines such as baby hamster kidney cells transformed with an expression vector encoding the recombinant molecule. The recombinant transferrins can be used in metal chelation therapy to bind and clear excess toxic metals in patients suffering from metal overloads or as tissue culture medium supplements or replacements.
摘要翻译： 描述了具有改变的金属结合或其它性质的重组转铁蛋白，非糖基化重组转铁蛋白，转铁蛋白半分子和突变体转运蛋白。 重组转铁蛋白分子以稳定的真核细胞系如用编码重组分子的表达载体转化的幼仓鼠肾细胞以功能形式表达。 重组转移蛋白可用于金属螯合治疗，以结合和清除患有金属超负荷或组织培养基补充剂或替代物的患者中的过量有毒金属。
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