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    PCR primers for detection and identification of plant pathogenic species, subspecies, and strains of acidovorax
    1.
    发明授权
    PCR primers for detection and identification of plant pathogenic species, subspecies, and strains of acidovorax 失效

    公开(公告)号：US06423499B1

    公开(公告)日：2002-07-23

    申请号：US09703807

    申请日：2000-11-02

    申请人： Wan-Yeob Song ,  Hyung-Moo Kim ,  Norman W. Schaad

    发明人： Wan-Yeob Song ,  Hyung-Moo Kim ,  Norman W. Schaad

    IPC分类号： C12Q168

    CPC分类号： C12Q1/689 ,  C12Q2561/101

    摘要： We sequenced a 619 and 617 bp fragment of the inner spacer region of 16S-23S rDNA of a strain of Acidovorax avenae representing pathogens from several hosts, including foxtail, oats, corn, rice, millet, sugarcane, orchid, and watermelon and a strain of A. avenae subsp. citrulli pathogenic only to watermelon and melons, respectively, for the purpose of designing PCR primers for their identification. These plant pathogens were previously considered as non-fluorescent pseudomonads and have been recently reclassified as Acidovorax avenae subsp. avenae, A. avenae subsp. cattleyae, and A. avenae subsp. citrulli. Several sets of primers were designed. Primers identified by SEQ ID NO:1 and SEQ ID NO:2 of A. avenae subsp. avenae reacted with all strains of A. avenae subsp. avenae (previously named P. avenae or P. alboprecipitans) originating from foxtail, oats, corn, rice, sugarcane, and millet, A. avenae subsp. cattleyae (previously named P. pseudoalcaligenes subsp. cattleyae) from orchid, and A. avenae subsp. citrulli (previously named P. pseudoalcaligenes subsp. citrulli) from watermelon and melon. Primers identified by SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 of subsp. citrulli reacted with all strains of A. avenae subsp. citrulli, but not with any other strain of A. avenae subsp. avenae. Primers identified by SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, reacted with the rice strains of A. avenae subsp. avenae, but not with any other strain of A. avenae subsp. avenae or any other subspecies of A. avenae. None of fifty-three other bacteria tested reacted with these sets of primers. The citrulli-specific primers and rice strain-specific primers should prove especially useful for the specific, sensitive, and rapid detection of these serious seedborne pathogens in watermelon and rice seeds, respectively.