公开(公告)号：US20220162543A1

公开(公告)日：2022-05-26

申请号：US17419122

申请日：2019-12-27

申请人： WILMAR (SHANGHAI) BIOTECHNOLOGY RESEARCH & DEVELOPMENT CENTER CO., LTD

发明人： Wei Wu ,  Xiaojun Dai ,  Haisheng Cao ,  Meifeng Zhou ,  Qiwen Niu

IPC分类号： C12N1/16 ,  C12N9/16 ,  C12N15/81 ,  C12P7/649

摘要： Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\′-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.

公开(公告)号：US11566204B2

公开(公告)日：2023-01-31

申请号：US16771447

申请日：2018-12-10

申请人： Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.

发明人： Wei Wu ,  Xiaojun Dai ,  Haisheng Cao ,  Qiwen Niu

IPC分类号： C11B3/00 ,  C12N9/16 ,  C12N15/81

摘要： According to the present disclosure, on the basis of all existing mutations, the tenth glycine of a BC-PC-PLC is mutated into aspartic acid, a specific enzyme activity thereof is 83% higher than that of a sequence before the mutation, and protein expression and degumming activity of unit enzyme activity do not change, so as to further reduce manufacturing costs.