公开(公告)号：US20120191364A1

公开(公告)日：2012-07-26

申请号：US13499265

申请日：2010-09-29

申请人： Wing Cheong Christopher Wong ,  Wah Heng Charlie Lee ,  Wing Kin Sung ,  Martin Lloyd Hibberd

发明人： Wing Cheong Christopher Wong ,  Wah Heng Charlie Lee ,  Wing Kin Sung ,  Martin Lloyd Hibberd

IPC分类号： G06F19/00 ,  C40B40/06

CPC分类号： G16B25/00 ,  G16B20/00

摘要： A method of sequencing a first polynucleotide strand having a first polynucleotide sequence, the first polynucleotide strand resembling a second polynucleotide strand having a known second polynucleotide sequence, the method employing a data set which, for one or more fragment(s) of the second polynucleotide sequence, contains: for each position along each said fragment: (i) first probe data describing the hybridization intensity of the first polynucleotide strand with a respective first probe designed to bind to a portion of the second polynucleotide strand centered at said position; and (ii) second probe data describing the respective hybridization intensities of the first polynucleotide strand with each of a set of second probes, each said second probe being designed to bind with a respective mutation of the corresponding portion of the second polynucleotide sequence which is formed by mutating the corresponding portion of the second polynucleotide sequence at said position, the data set including said second probe data for every possible said mutation; the method comprising: for each said position, obtaining from the dataset a first numerical parameter characterizing the hybridization intensity of the first polynucleotide strand with the corresponding first probe in comparison to the hybridization intensities of the first polynucleotide strand with the corresponding second probes; said first numerical parameter being indicative of whether a nucleic acid of the first polynucleotide sequence is equal to a nucleic acid the second polynucleotide sequence at said position.

摘要翻译： 测序具有第一多核苷酸序列的第一多核苷酸链的方法，所述第一多核苷酸链与所述第二多核苷酸序列相似，所述第一多核苷酸链类似具有已知第二多核苷酸序列的第二多核苷酸链，所述方法采用数据集，所述数据集针对所述第二多核苷酸 序列包含：沿着每个所述片段的每个位置：（i）首先探测数据，其描述第一多核苷酸链的杂交强度与设计成结合至位于所述位置的第二多核苷酸链的一部分的相应第一探针; 和（ii）描述第一多核苷酸链与一组第二探针中的每一个的相应杂交强度的第二探针数据，每个所述第二探针设计成与形成的第二多核苷酸序列的相应部分的相应突变结合 通过在所述位置突变所述第二多核苷酸序列的相应部分，所述数据集包括针对每个可能的所述突变的所述第二探针数据; 所述方法包括：对于每个所述位置，与所述第一多核苷酸链与相应的第二探针的杂交强度相比，从所述数据集获得表征所述第一多核苷酸链与相应的第一探针的杂交强度的第一数值参数; 所述第一数值参数指示第一多核苷酸序列的核酸是否等于所述位置处的第二多核苷酸序列的核酸。