公开(公告)号：US20210024901A1

公开(公告)日：2021-01-28

申请号：US16825652

申请日：2020-03-20

Applicant: XIAMEN UNIVERSITY

Inventor： Keju JING ,  Yujiao SHEN ,  Wei XIONG ,  Xueping LING ,  Yinghua LU

IPC: C12N9/04 ,  C12N15/10

Abstract: The present invention provides a method for changing an coenzyme activity and a preference of glucose dehydrogenase and the use thereof, and relates to the technical field of genetic engineering. In the method of the present invention, by performing a site-directed mutation of the fourth amino acid in a conserved sequence GXXXGXG of the glucose dehydrogenase, a mutant protein with the changed coenzyme activity and preference can be simply and directly obtained without a large amount of screening, such that the enzyme can be widely applied to catalyze a coenzyme regeneration reaction. In the embodiments of the present invention, four glucose dehydrogenase mutants T17G, T17K, T17RGDH DN46 and K17G are finally obtained by site-directed mutation of the 4th amino acid (which binds with a nicotinamide coenzyme ribose 2′-phosphate) in the conserved sequence GXXXGXG widely existed in glucose dehydrogenases from different sources, and upon verification, the enzyme activity, preference and the like of them all have been varied.

公开(公告)号：US20200239646A1

公开(公告)日：2020-07-30

申请号：US16374464

申请日：2019-04-03

Applicant: XIAMEN UNIVERSITY

Inventor： Liming CHE ,  Xiang LI ,  Ning HE ,  Xue'e WU ,  Yuanpeng WANG ,  Xueping LING

IPC: C08J3/075 ,  A61L27/52 ,  A61L27/20 ,  A61K47/36 ,  A61K35/747 ,  A61K31/353 ,  A61K38/38 ,  A61K31/375

Abstract: The invention relates to a method for embedding a load based on gel high hydrostatic pressure liquefaction. Using the phenomenon that the physical gel is liquefied under high pressure, the vacuum-packaged high-methoxyl pectin gel is treated under a pressure of 400-600 MPa for 5-30 min, mixed with the load, and then subjected to a pressure of 400-600 MPa for homogenization treatment for 5 to 30 min After pressure relief, the liquefied gel is poured into a mold for reshaping, followed by removal of free water and coating treatment. This method combines the advantages of high hydrostatic pressure technology in modification and sterilization. It has mild embedding conditions and wide sources of raw materials to prepare the carrier, which has excellent biocompatibility and biodegradability. It can be widely used for embedding microorganisms, enzymes, proteins and small molecular substances. The loaded gel prepared by the method has high microbial safety, can effectively maintain the activity of the load. The load distribution is uniform, and the load amount is much larger than the traditional adsorption load.