公开(公告)号：US20140178869A1

公开(公告)日：2014-06-26

申请号：US13843408

申请日：2013-03-15

申请人： Xiao-Jun MA ,  Yuling LUO ,  Raymond Tubbs

发明人： Xiao-Jun MA ,  Yuling LUO ,  Raymond Tubbs

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C07K16/00 ,  C07K2317/515 ,  C12Q1/6886 ,  C12Q2600/158

摘要： The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by obtaining a sample of B cells from a subject; conducting a duplex in situ hybridization assay on the sample using (i) at least one probe set which is designed to specifically hybridize to immunoglobulin kappa chain constant region (IGKCR) RNA; and (ii) at least one probe set which is designed to specifically hybridize to immunoglobulin lambda chain constant region (IGLCR) RNA; detecting signal associated with hybridized IGKCR probe and signal associated with hybridized IGLCR probe in a population of B cells in the sample; and determining a pattern of signal associated with hybridized IGKCR probe and hybridized IGLCR probe within individual cells in the B cell population, wherein the pattern of signal within individual cells indicates the presence or absence of light chain restriction and clonality of the B cells.

摘要翻译： 本发明提供一种通过从受试者获得B细胞样品来检测B细胞中免疫球蛋白轻链限制和克隆性的方法; 使用（i）设计为与免疫球蛋白κ恒定区（IGKCR）RNA特异性杂交的至少一个探针组进行样品上的双链原位杂交测定; 和（ii）设计成与免疫球蛋白λ链恒定区（IGLCR）RNA特异性杂交的至少一个探针组; 检测与杂交的IGKCR探针相关的信号和与样品中B细胞群中的杂交IGLCR探针相关的信号; 以及确定在B细胞群体中的各个细胞内与杂交的IGKCR探针和杂交的IGLCR探针相关联的信号模式，其中各个细胞内的信号模式指示存在或不存在B细胞的轻链限制和克隆。