公开(公告)号：US20110081708A1

公开(公告)日：2011-04-07

申请号：US12894401

申请日：2010-09-30

申请人： Xiaowu LIU ,  Yun HE ,  Zhuying WANG ,  Chunjiao WANG ,  Zhibing LIU ,  Tianhui XIA ,  Luquan WANG ,  Fang Liang ZHANG

发明人： Xiaowu LIU ,  Yun HE ,  Zhuying WANG ,  Chunjiao WANG ,  Zhibing LIU ,  Tianhui XIA ,  Luquan WANG ,  Fang Liang ZHANG

IPC分类号： C12N1/21 ,  C07H21/04 ,  C12N15/63 ,  G06F19/00

CPC分类号： G06F19/22 ,  G06F19/20

摘要： An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

摘要翻译： 描述了改进的基因序列优化方法，该系统优化方法用于增强细菌，酵母，昆虫和哺乳动物细胞中基因的重组表达。 这种一般方法考虑到影响蛋白质表达的参数和因子的多个，优选大多数或全部，包括密码子使用，tRNA使用，GC含量，核糖体结合序列，启动子，5'-UTR，ORF和3'-UTR 改进和优化基因序列以增强细菌，酵母，昆虫和哺乳动物细胞中基因的蛋白质表达的基因序列。 特别地，本发明涉及使用粒子群优化算法改进重组蛋白表达的序列优化的系统和方法。 改进的系统优化方法可以被并入软件中以进行更有效的优化。