公开(公告)号：US20150211041A1

公开(公告)日：2015-07-30

申请号：US14421834

申请日：2013-08-14

Applicant: YEDA RESEARCH AND DEVELOPMENT CO., LTD.

Inventor： Ehud Y. Shapiro ,  Tom Ran ,  Yehonatan Douek

IPC: C12Q1/02 ,  C12N15/63

CPC classification number: C12Q1/02 ,  C12N15/63 ,  C12N15/65 ,  C12N15/72 ,  C12N15/73

Abstract: An autonomous synthetic programmable device adapted to determine a cell state according to one or more different predefined markers, comprising a transcription machinery and a regulatory element for regulating said transcription machinery, said regulatory element comprising at least three different binding regions, each binding region binding a different transcription factor and each binding region corresponding to a different predefined marker, wherein the regulatory element permits said transcription machinery to provide a transcription output if and only if all three different transcription factors are absent and wherein each transcription factor is capable of blocking transcription by said transcription machinery and hence blocking said transcription output.

Abstract translation: 一种自适应合成可编程装置，其适于根据一种或多种不同的预定义标记确定细胞状态，所述标记包含转录机制和用于调节所述转录机制的调节元件，所述调节元件包含至少三个不同的结合区，每个结合区结合 不同的转录因子和对应于不同的预定标记的每个结合区域，其中所述调节元件允许所述转录机制提供转录输出，如果并且仅当所有三种不同的转录因子都不存在并且其中每个转录因子能够通过所述转录因子阻断转录 转录机制，从而阻止转录输出。