公开(公告)号：US20170045522A1

公开(公告)日：2017-02-16

申请号：US15307011

申请日：2015-04-28

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David MARGULIES ,  Leila MOTIEI ,  Naama MANKOVSKI ,  Yael NISSINKORN

IPC: G01N33/58 ,  G01N33/68

Abstract: This invention is directed to monomolecular sensors, comprising a selective binder, a non-selective binder and a fluorophore, which can track changes that occur on the surface of labeled proteins. This invention is further directed to the use of such sensors for identifying binding partners of specific proteins. This invention is further directed to His-tag binding compounds and uses thereof in the preparation of genetically targeted detectable molecules and sensors which can specifically bind tag-labeled proteins.

Abstract translation: 本发明涉及单分子传感器，其包括选择性粘合剂，非选择性粘合剂和荧光团，其可以跟踪标记蛋白质表面上发生的变化。 本发明还涉及这种传感器用于鉴定特定蛋白质的结合伴侣的用途。 本发明进一步涉及His-标签结合化合物及其在制备可特异性结合标签标记的蛋白质的遗传可靶向可检测分子和传感器的用途。