公开(公告)号：US06797483B1

公开(公告)日：2004-09-28

申请号：US10019455

申请日：2001-12-28

申请人： Yasuaki Itoh ,  Kazunori Nishi ,  Kazuhiro Ogi ,  Shoichi Ohkubo ,  Shinichi Mogi ,  Yuko Noguchi ,  Koji Yoshimura ,  Hideyuki Tanaka

发明人： Yasuaki Itoh ,  Kazunori Nishi ,  Kazuhiro Ogi ,  Shoichi Ohkubo ,  Shinichi Mogi ,  Yuko Noguchi ,  Koji Yoshimura ,  Hideyuki Tanaka

IPC分类号： G01N3353

CPC分类号： C07K14/4703 ,  A61K38/00

摘要： The present invention relates to novel secretory polypeptides and precursor proteins thereof, which show a high homology to MIA (melanoma inhibitor activity)/CD-RAP (bovine cartilage-derived retinoic acid-sensitive protein); are specifically expressed in the cartilage tissue; and have an effect on the cartilage differentiation. The polypeptides and the DNAs encoding the same can be used for the diagnosis, treatment, or prevention of bone and joint diseases or pathological angiogenesis. As well, the polypeptides are useful as a reagent for screening a compound that promotes or inhibits the activity of the polypeptides. The antibody to the polypeptides can be used for the detection, quantification, or neutralization of the polypeptide in a test sample fluid. Further by using the promoter of the present invention, a therapeutic protein can be expressed predominantly in the cartilage tissue for a gene therapy.

摘要翻译： 本发明涉及与MIA（黑素瘤抑制剂活性）/ CD-RAP（牛软骨衍生的视黄酸敏感蛋白）高同源性的新型分泌多肽及其前体蛋白; 在软骨组织中特异性表达; 并对软骨分化有影响。 编码该多肽和DNA的DNA可用于诊断，治疗或预防骨和关节疾病或病理性血管生成。 同样，多肽可用作筛选促进或抑制多肽活性的化合物的试剂。 多肽的抗体可用于在测试样品流体中检测，定量或中和多肽。 此外，通过使用本发明的启动子，治疗性蛋白质主要在软骨组织中表达用于基因治疗。

公开(公告)号：US20060073516A1

公开(公告)日：2006-04-06

申请号：US10509484

申请日：2003-03-27

申请人： Yasuaki Ito ,  Tokuyuki Shinohara ,  Masaki Hosoya ,  Shuji Hinuma ,  Yuko Noguchi

发明人： Yasuaki Ito ,  Tokuyuki Shinohara ,  Masaki Hosoya ,  Shuji Hinuma ,  Yuko Noguchi

IPC分类号： G01N33/53 ,  A61K38/17 ,  C07H21/04 ,  C12P21/06 ,  A61K31/195 ,  C07K14/705

CPC分类号： C07K14/705

摘要： Using a novel G protein-coupled receptor protein having an amino acid sequence which is the same or substantially the same amino acid sequence as an amino acid sequence represented by SEQ ID NO: 2 or its salt together with β-alanine or L-carnosine, an agonist or an antagonist to the receptor protein or its salt can be efficiently screened.

摘要翻译： 使用具有与SEQ ID NO：2表示的氨基酸序列相同或基本相同的氨基酸序列的氨基酸序列的新型G蛋白偶联受体蛋白或其盐与β-丙氨酸或L-肌肽一起使用， 可以有效地筛选受体蛋白质或其盐的激动剂或拮抗剂。