公开(公告)号：US20240352538A1

公开(公告)日：2024-10-24

申请号：US18764250

申请日：2024-07-04

申请人： Yeda Research and Development Co. Ltd. ,  SeqLL Inc.

发明人： Efrat SHEMA-YAACOBY ,  Erez NIR ,  Vadim FEDYUK ,  Ekaterina ANDREISHCHEVA ,  Daniel JONES ,  Abhijeet SHINDE

IPC分类号： C12Q1/6886 ,  C12Q1/6804

CPC分类号： C12Q1/6886 ,  C12Q1/6804 ,  C12Q2600/156

摘要： A method of analyzing nucleosomes is provided. The method comprising: (a) isolating a plurality of nucleosome molecules from a biological sample; (b) enzymatically linking adenine nucleotides to free DNA ends of the plurality of nucleosome molecules, wherein at least a portion of the adenine nucleotides comprises a label, such that the plurality of nucleosome molecules become attached to a labeled poly(A) tail; (c) hybridizing the plurality of nucleosome molecules attached to the labeled poly(A) tail to a solid support coated with poly(T); and (d) imaging the solid support, whereby the plurality of nucleosome molecules are visualized.