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1.发明授权公开(公告)号：US07601807B2
公开(公告)日：2009-10-13
申请号：US11287307
申请日：2005-11-28
申请人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
IPC分类号： C07K1/00
CPC分类号： C12N9/52
摘要： A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: (1) (SEQ ID NO:4) Pro-Gln-Gly-Phe-Gln-Gly-Pro (2) (SEQ ID NO:5) Pro-Ala-Gly-Phe-Ala-Gly-Pro (3) (SEQ ID NO:6) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
摘要翻译： 具有以下特征的蛋白酶：（i）分子量为21,000±1,000（通过SDS-PAGE）和（ii）切割包含以下氨基酸序列（1）的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性， 切割包含以下氨基酸序列（2）的肽或蛋白质的Gly-Phe位点，或切割包含以下氨基酸序列（3）的肽或蛋白质的Ser-Leu位点：（1）（SEQ ID NO：4 ）Pro-Gln-Gly-Phe-Gln-Gly-Pro（2）（SEQ ID NO：5）Pro-Ala-Gly-Phe-Ala-Gly-Pro（3）（SEQ ID NO：6）Gln-Thr -Gln-Ser-Leu-Val-Thr-Pro。 编码该蛋白酶的DNA，包括含有该DNA的载体，以及通过用该DNA转化细胞来生产该蛋白酶的方法。
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2.发明授权Method for manufacturing gelatin with reduced endotoxin content and low endotoxin gelatin 有权降低内毒素含量和低内毒素明胶制造明胶的方法公开(公告)号：US07855273B2
公开(公告)日：2010-12-21
申请号：US11681323
申请日：2007-03-02
申请人： Yoshitaka Kanayama , Yasuo Sakai
发明人： Yoshitaka Kanayama , Yasuo Sakai
CPC分类号： A61L27/222 , A61K9/0024 , C08H1/06 , C09H3/02
摘要： A method of manufacturing gelatin with reduced endotoxin content. The method comprises processing a starting material gelatin-containing solution containing gelatin and an endotoxin with an ultrafiltration film having a molecular weight cut-off falling within a range of from 20,000 to 300,000 and having a molecular weight cut-off capable of passing at least a portion of the gelatin contained in the starting material gelatin-containing solution to obtain a permeate that is a gelatin-containing solution with a reduced endotoxin content. A gelatin having an average molecular weight falling within a range of 1,000 to 300,000 and an endotoxin content of less than 1 EU/mL per 1.0 percent of protein.
摘要翻译： 制造具有降低的内毒素含量的明胶的方法。 该方法包括用分子量截留值为20,000〜300,000的超滤膜加工含有明胶和内毒素的原料明胶溶液，并具有分子量截留能够通过至少一种 含有起始原料明胶溶液中的明胶的一部分，以获得具有降低的内毒素含量的含有明胶的溶液的渗透物。 平均分子量在1000至300,000范围内，每1.0％蛋白质的内毒素含量小于1EU / mL的明胶。
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3.发明申请公开(公告)号：US20060134749A1
公开(公告)日：2006-06-22
申请号：US11287307
申请日：2005-11-28
申请人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
CPC分类号： C12N9/52
摘要： The present invention provides a protease, DNA encoding this protease, a recombinant vector comprising this DNA, and a method for manufacturing the protease using the transformant.
摘要翻译： 本发明提供蛋白酶，编码该蛋白酶的DNA，包含该DNA的重组载体，以及使用该转化体制造蛋白酶的方法。
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公开(公告)号：US07871797B2
公开(公告)日：2011-01-18
申请号：US12537259
申请日：2009-08-07
申请人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
发明人： Yoshitaka Kanayama , Yasuo Sakai , Takahiro Hongo
IPC分类号： C12P21/02
CPC分类号： C12N9/52
摘要： The present invention relates to a protease having a molecular weight of 21,000±1,000 (by SDS-PAGE) and a specific protease activity capable of cutting at the Gly-Phe site of a peptide or protein comprising the amino acid sequence Pro-Gln-Gly-Phe-Gln-Gly-Pro or Pro-Ala-Gly-Phe-Ala-Gly-Pro or cutting at the Ser-Leu site of a peptide or protein comprising the amino acid sequence Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. The invention further provides DNA encoding this protease, as well as vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.
摘要翻译： 本发明涉及分子量为21,000±1,000（通过SDS-PAGE）的蛋白酶和能够在包含氨基酸序列Pro-Gln-Gly的肽或蛋白质的Gly-Phe位点切割的特异性蛋白酶活性 -Phe-Gln-Gly-Pro或Pro-Ala-Gly-Phe-Ala-Gly-Pro或在包含氨基酸序列Gln-Thr-Gln-Ser-Leu- Val-Thr-Pro。 本发明还提供了编码该蛋白酶的DNA，以及含有该DNA的载体，以及通过用该DNA转化细胞来生产该蛋白酶的方法。
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5.发明申请METHOD FOR MANUFACTURING GELATIN WITH REDUCED ENDOTOXIN CONTENT AND LOW ENDOTOXIN GELATIN 有权降低内毒素含量和低内毒素凝胶的制备方法公开(公告)号：US20080132682A1
公开(公告)日：2008-06-05
申请号：US11681323
申请日：2007-03-02
申请人： Yoshitaka Kanayama , Yasuo Sakai
发明人： Yoshitaka Kanayama , Yasuo Sakai
CPC分类号： A61L27/222 , A61K9/0024 , C08H1/06 , C09H3/02
摘要： A method of manufacturing gelatin with reduced endotoxin content. The method comprises processing a starting material gelatin-containing solution containing gelatin and an endotoxin with an ultrafiltration film having a molecular weight cut-off falling within a range of from 20,000 to 300,000 and having a molecular weight cut-off capable of passing at least a portion of the gelatin contained in the starting material gelatin-containing solution to obtain a permeate that is a gelatin-containing solution with a reduced endotoxin content. A gelatin having an average molecular weight falling within a range of 1,000 to 300,000 and an endotoxin content of less than 1 EU/mL per 1.0 percent of protein.
摘要翻译： 制造具有降低的内毒素含量的明胶的方法。 该方法包括用分子量截留值为20,000〜300,000的超滤膜加工含有明胶和内毒素的原料明胶溶液，并具有分子量截留能够通过至少一种 含有起始原料明胶溶液中的明胶的一部分，以获得具有降低的内毒素含量的含有明胶的溶液的渗透物。 平均分子量在1000至300,000范围内，每1.0％蛋白质的内毒素含量小于1EU / mL的明胶。
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6.发明授权Method of improving quality of mortar or concrete structures and additives therefor 失效提高砂浆或混凝土结构及添加剂质量的方法
公开(公告)号：US4986854A
公开(公告)日：1991-01-22
申请号：US400807
申请日：1989-08-30
申请人： Masahiko Okuno , Yoshitaka Kanayama
发明人： Masahiko Okuno , Yoshitaka Kanayama
摘要： Disclosed is a method of improving the quality of a structure to be prepared from mortar or concrete, the method being characterized in that when the mortar or concrete is kneaded, a water-soluble amino resin is admixed therewith, or a water soluble amino resin and at least one of a higher fatty acid metal salt and a nonionic surfactant are admixed therewith.
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