公开(公告)号：US20060094083A1

公开(公告)日：2006-05-04

申请号：US11211464

申请日：2005-08-26

申请人： Yun-Jaie Choi ,  Chang-Hoon Lee ,  Jung-Hee Woo ,  Seung-Ha Kang ,  Seung-Hoon Choi ,  Sang-Kee Kang

发明人： Yun-Jaie Choi ,  Chang-Hoon Lee ,  Jung-Hee Woo ,  Seung-Ha Kang ,  Seung-Hoon Choi ,  Sang-Kee Kang

IPC分类号： C12P21/06 ,  C12N1/21 ,  C12N1/18 ,  C07K14/61

CPC分类号： C07K14/61 ,  C07K2319/30 ,  C12N15/746 ,  C12N15/815

摘要： The present invention relates to probiotic microorganisms producing chimeric human growth hormone for oral use and methods for preparing them. The invention provides probiotic Lactobacillus or yeast transformant expressing chimeric protein which is human growth hormone fused with Fc fragment of human IgG, in which the transformants are safely delivered into intestine though oral route. Also, the invention provides a chimeric protein-expressing vector which can induce transcytosis in intestine epithelial cells. Accordingly, the invention demonstrates that the chimeric protein for oral delivery system can be absorbed in intestine, and delivery of the chimeric protein by oral route using Lactobacillus has very excellent efficiency in vivo test in rats. Accordingly, the Lactobacillus of the present invention is an excellent deliverer of protein drugs.

摘要翻译： 本发明涉及产生用于口服的嵌合人生长激素的益生菌微生物及其制备方法。 本发明提供了表达嵌合蛋白的益生菌乳杆菌或酵母转化体，其是与人IgG的Fc片段融合的人生长激素，其中转化体通过口服途径安全递送至肠。 此外，本发明提供了可诱导肠上皮细胞转胞吞作用的嵌合蛋白质表达载体。 因此，本发明表明用于口服递送系统的嵌合蛋白质可以在肠中被吸收，并且使用乳杆菌通过口服途径递送嵌合蛋白质具有非常优异的大鼠体内试验效率。 因此，本发明的乳杆菌是蛋白质药物的优良递送剂。