公开(公告)号：US20240027428A1

公开(公告)日：2024-01-25

申请号：US18182377

申请日：2023-03-13

Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY

Inventor： Rongfa Guan ,  Mengyu Yang ,  Hao Zhong ,  Xiaofeng Liu ,  Donghong Liu ,  Guozhou Cao ,  Yong Cheng ,  Haitao Shen ,  Jun Huang ,  Kai Yang ,  Yanbin Wang ,  Haizhi Huang

IPC: G01N33/50 ,  C12N5/09

CPC classification number: G01N33/5011 ,  C12N5/0693 ,  C12N2500/36 ,  C12N2500/34 ,  C12N2502/30

Abstract: The present invention discloses a method for building a co-culture model for Caco-2/RAW-264.7 cells induced by lipopolysaccharides and an application of the co-culture model. The method includes: 1) culturing Caco-2 cells and RAW-264.7 cells; 2) inoculating the 5 Caco-2 cells onto an AP side of an upper chamber of a Transwell plate, and incubating; 3) inoculating the RAW-264.7 cells onto a 24-well plate, and incubating; 4) transferring the upper chamber of the Transwell plate into the 24-well plate; 5) dissolving lipopolysaccharides in PBS to prepare a stock solution, filtering, and diluting for later use; and 6) adding an LPS-10% DMEM medium on a BL side, incubating, and forming an intestinal inflammation model.