公开(公告)号：US20230295752A1

公开(公告)日：2023-09-21

申请号：US18058851

申请日：2022-11-25

Applicant: ZHENGZHOU UNIVERSITY

Inventor： Aiping WANG ,  Gaiping ZHANG ,  Haili WANG ,  Yuhang ZHANG ,  Yumei CHEN ,  Jingming ZHOU ,  Ying ZHANG ,  Xifang ZHU ,  Chao LIANG

IPC: C12Q1/70 ,  G01N33/543

CPC classification number: C12Q1/707 ,  G01N33/54388 ,  G01N2333/02

Abstract: The present disclosure disclosed recombinase aided amplification (RAA) primers and kits for the detection of hepatitis c virus. Nucleotide sequences of the RAA primers include: an upstream primer: 5′-FITC-CTTGGGATATGATGATGAACTGGTCACCTAC-3′ (SEQ ID NO. 1); and a downstream primer: 5′-Biotin-AAGAGTAGCATCACAATCAGAACCTTAGCC-3′ (SEQ ID NO. 2). The HCV detection by using the RAA primers screened by the present disclosure has good specificity and high sensitivity (at least 10 copies/μL can be detected). The RAA primers can be used to prepare an HCV detection kit and construct an RAA amplification system. And combined with a lateral flow chromatography technology, the present disclosure can achieve rapid and low-cost detection of HCV and visual result judgment, no complex professional background is required, the use process is convenient and fast, and the detection results are safe and reliable.