专利检索 ap:("Zhejiang Difference Biological Technology Co., Ltd.") AND inv:"Ruiting CHEN" 第 1 页

1.

发明公开
METHOD FOR ATTENUATING INFLUENZA VIRUS BY SYNONYMOUS MUTATION AND DELETION MUTATION, AND ATTENUATED INFLUENZA VIRUS STRAIN AND USE 审中-公开

公开(公告)号：US20240352442A1

公开(公告)日：2024-10-24

申请号：US18137786

申请日：2023-04-21

申请人： Zhejiang Difference Biological Technology Co., Ltd.

发明人： Shuihua MAO , Mengyun ZHOU , Ruiting CHEN , Xiaofei LIU , Jiasheng SONG

IPC分类号： C12N15/10 , C12N7/04

CPC分类号： C12N15/102 , C12N7/04 , C12N2760/16121

摘要： The present disclosure relates to a method for attenuating an influenza virus, an attenuated influenza virus strain and use thereof, and belongs to the technical field of attenuated vaccines. In the present disclosure, the method includes the following steps: conducting synonymous mutation on an overlap of an M2 gene and an M1 gene in an M gene of an influenza A virus, while ensuring integrity and invariance of an amino acid sequence encoded by the M1 gene; conducting mutation into termination codon and deletion of partial nucleotide sequence in a transmembrane domain of the M2 gene, and rescuing an attenuated influenza virus strain using a reverse genetic system.