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公开(公告)号：US20210332157A1
公开(公告)日：2021-10-28
申请号：US17010808
申请日：2020-09-02
Applicant: Zhejiang University of Technology
Inventor： Ping Shao , Peiyu Qian , Jiefeng Pan , Peilong Sun
Abstract: A method for extracting and purifying Dendrobium officinale polysaccharides comprises following steps: (1) fully disperse Dendrobium officinale powder in pure water to obtain crude liquid; (2) removing insoluble impurities from the crude liquid through a microfiltration membrane to obtain permeate 1 and retentate 1; (3) performing macroporous ultrafiltration treatment of the permeate 1 and collect permeate 2 and retentate 2; (4) adding an aqueous solution of edible alkali metal inorganic salt to the retentate 2, fully stirring and dissolving to obtain polysaccharide crude liquid, performing macroporous ultrafiltration treatment and collecting permeate 3 and retentate 3; (5) combining the permeate 2 and permeate 3, adding the combined permeate into an electrodialysis device for desalination, and collecting dilute solution and concentrated solution; (6) performing microporous ultrafiltration treatment of the dilute solution and collect retentate 4 and permeate 4; (7) carrying out freeze-drying of the retentate 4 to obtain Dendrobium officinale polysaccharides.
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公开(公告)号：US11780936B2
公开(公告)日：2023-10-10
申请号：US17010808
申请日：2020-09-02
Applicant: Zhejiang University of Technology
Inventor： Ping Shao , Peiyu Qian , Jiefeng Pan , Peilong Sun
CPC classification number: C08B37/0003 , B01D61/145 , B01D61/146 , B01D61/147 , B01D61/422 , B01D61/58 , B01D69/02 , B01D2311/12 , B01D2317/025 , B01D2325/20
Abstract: A method for extracting and purifying Dendrobium officinale polysaccharides comprises following steps: (1) fully disperse Dendrobium officinale powder in pure water to obtain crude liquid; (2) removing insoluble impurities from the crude liquid through a microfiltration membrane to obtain permeate 1 and retentate 1; (3) performing macroporous ultrafiltration treatment of the permeate 1 and collect permeate 2 and retentate 2; (4) adding an aqueous solution of edible alkali metal inorganic salt to the retentate 2, fully stirring and dissolving to obtain polysaccharide crude liquid, performing macroporous ultrafiltration treatment and collecting permeate 3 and retentate 3; (5) combining the permeate 2 and permeate 3, adding the combined permeate into an electrodialysis device for desalination, and collecting dilute solution and concentrated solution; (6) performing microporous ultrafiltration treatment of the dilute solution and collect retentate 4 and permeate 4; (7) carrying out freeze-drying of the retentate 4 to obtain Dendrobium officinale polysaccharides.
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