Computerized method of matching two-dimensional (2-D) patterns
    2.
    发明授权
    Computerized method of matching two-dimensional (2-D) patterns 失效
    匹配二维(2-D)图案的计算方法

    公开(公告)号:US5073963A

    公开(公告)日:1991-12-17

    申请号:US528816

    申请日:1990-05-25

    CPC classification number: G06K9/6203 G01N27/44721

    Abstract: This invention teaches a computerized method for use in data acquisition and manipulation of two-dimensional patterns in the fields of medicine, astronomy, chemistry, biology and biotechnology. The invention teaches an interactive computerized method for matching visual patterns of polypeptide spots in two-dimensional (2-D) gel electrophoretogram solubilized into polypeptide constituents that are separated by electrophoresis. The computerized method manipulates spot pixel coordinates using staged coordinate transformation techniques on spot markers and unknown study spots to reduce gel preparation distortions and allows a user to produce matching results in a manner that compares the transformed spot data using either a single reference gel or multiple reference gels approach for producing the matching results. The method also includes a spot matching verification step and a step to extract potentially mis-matched spots from reported matching results. The user can also resolve contradictions and perform spot matching analysis using isoelectric focusing (PI), and molecular weight (MW) dimensional separation data.

    Abstract translation: 本发明教导了一种用于医学,天文学,化学,生物学和生物技术领域的二维图案的数据采集和操作的计算机化方法。 本发明教导了一种交互式计算机化方法,用于匹配溶解成通过电泳分离的多肽成分的二维(2-D)凝胶电泳图中的多肽点的视觉模式。 计算机化方法使用分段坐标转换技术在斑点标记和未知研究点上操纵斑点像素坐标,以减少凝胶制备失真,并允许用户以使用单个参考凝胶或多个参考比较转化的斑点数据的方式产生匹配结果 凝胶法生产匹配结果。 该方法还包括点匹配验证步骤和从报告的匹配结果中提取潜在的不匹配斑点的步骤。 用户还可以解决矛盾,并使用等电聚焦(PI)和分子量(MW)尺寸分离数据进行匹配分析。

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