公开(公告)号：US5637496A

公开(公告)日：1997-06-10

申请号：US508618

申请日：1995-07-28

申请人： Thomas Thaler ,  Gerhard Klement

发明人： Thomas Thaler ,  Gerhard Klement

IPC分类号： C12M1/26 ,  C12M3/00

CPC分类号： C12M27/02 ,  C12M33/10 ,  C12M47/02

摘要： The device for conveying and separating a suspension with biological cells or micro-organisms in biological processes has a rotating separating element in a surrounding reaction space with a flow space which is in the form of a rotational gap, an inlet opening, an inner outlet opening for the cell-free medium, an outer through opening and an exit opening for the suspension. The distance R4 of the exit opening from the axis of rotation is greater than the distance R1 of the inlet opening, and the distance R3 of the through opening is greater than the distance R2 of the inner outlet opening. The suspension can thus be conveyed in the flow space and the cells efficiently separated at the same time. The device is particularly suitable for continuous operation with sensitive mammal cells.

摘要翻译： 用于在生物过程中用生物细胞或微生物输送和分离悬浮液的装置在周围的反应空间中具有旋转的分离元件，其具有旋转间隙形式的流动空间，入口开口，内部出口开口 对于无细胞介质，用于悬浮液的外部通孔和出口。 出口开口距转轴的距离R4大于入口开口的距离R1，通孔的距离R3大于内出口开口的距离R2。 因此，悬浮液可以在流动空间中传送并且细胞同时有效地分离。 该装置特别适用于与敏感哺乳动物细胞的连续操作。