公开(公告)号：US20050026247A1

公开(公告)日：2005-02-03

申请号：US10734583

申请日：2003-12-15

申请人： Hidetoshi Andou ,  Jun Honda ,  Shunjiro Sugimoto ,  Gertrud Hotten ,  Rolf Bechtold ,  Jens Pohl

发明人： Hidetoshi Andou ,  Jun Honda ,  Shunjiro Sugimoto ,  Gertrud Hotten ,  Rolf Bechtold ,  Jens Pohl

IPC分类号： A61K38/00 ,  C07K14/475 ,  C07K14/51 ,  C07K14/575 ,  C12N1/21 ,  C07H21/04

CPC分类号： C07K14/51 ,  A61K38/00 ,  C07K14/475 ,  C07K14/575

摘要： A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.

摘要翻译： 一种制备骨源性因子的纯化的重折叠单体或二聚体的方法，其包括使基因工程产生的骨衍生因子的内含体按顺序步骤a）至c）：a）将多核苷酸 将骨形态发生因子编码成细菌，以包涵体的形式表达所述骨形态发生因子，回收所述包涵体并用变性剂处理以获得溶解的单体，b）直接用 重折叠溶液以获得重折叠的单体骨形态发生因子，c）使重折叠的单体骨形态发生因子进行纯化。