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    Evaporation apparatus and method
    1.
    发明授权
    Evaporation apparatus and method 有权

    公开(公告)号：US11577178B2

    公开(公告)日：2023-02-14

    申请号：US16093817

    申请日：2017-04-25

    申请人： Biotage AB

    发明人： Adam Faris ,  Johan Asplund ,  Olof Eriksson

    IPC分类号： B01D1/12 ,  B01D1/00 ,  B01D1/14 ,  G01N1/40 ,  B01D3/42 ,  B01D3/34

    摘要： The present invention relates to evaporation apparatus (100) comprising manifolds provided with at least one nozzle (102), a tank unit (103) for a liquid, and a sample holder configured to be inserted into the tank unit. The sample holder is configured to hold at least one sample in a defined position relative the at least one nozzle a control unit (104) an inlet port (105) configured to be connected to a gas supply, a pressure regulator (106) arranged downstream the inlet port (105). A set value of the pressure regulator (106) is controlled by the control unit (104), a control valve (107) arranged downstream the pressure regulator (106), wherein each of the at least one manifold (101a-d) is connected to a corresponding output port of the control valve. The control valve is controlled by the control unit (104), and the control unit is configured to set the set value of the pressure regulator to a value that causes a predetermined gas flow from each of the at least one nozzle.

    MASS-DIRECTED SEPARATION
    2.
    发明申请
    MASS-DIRECTED SEPARATION 审中-公开

    公开(公告)号：US20200041472A1

    公开(公告)日：2020-02-06

    申请号：US16484649

    申请日：2018-03-14

    申请人： Biotage AB

    发明人： Anders WARNGREN

    IPC分类号： G01N30/72 ,  G01N30/86

    摘要： The present invention is a method for automated mass-directed separation of two or more components from a sample, which method comprises defining a solvent gradient by its changing composition; subjecting said solvent gradient to mass spectrometry (MS) to generate gradient signal(s); passing a gradient including at least part(s) of the defined gradient across a packed chromatography column to which a sample has been applied; subjecting the eluent exiting said column to MS to generate sample signal(s); generating a spectrum by subtracting the gradient signals from the sample signals across a selected range of m/z values; and directing a fraction collector to collect fraction(s) each comprising a separated component based on the spectrum generated.

    Vessel for performing microwave-assisted chemistry on small volumes of reagents
    5.
    发明授权
    Vessel for performing microwave-assisted chemistry on small volumes of reagents 有权
    用于在小体积试剂上进行微波辅助化学的容器

    公开(公告)号：US08609044B2

    公开(公告)日：2013-12-17

    申请号：US10539045

    申请日：2003-12-18

    申请人： Johan Ulin ,  Per-Gunnar Eriksson ,  Fredrik Ekdahl

    发明人： Johan Ulin ,  Per-Gunnar Eriksson ,  Fredrik Ekdahl

    IPC分类号： B01L3/14

    CPC分类号： B01J19/126 ,  B01J19/0093 ,  B01J2219/00788 ,  B01J2219/00941 ,  B01J2219/1215 ,  B01J2219/1227 ,  B01J2219/1233 ,  B01J2219/1236 ,  B01J2219/1254 ,  B01L3/5082 ,  B01L2300/042 ,  B01L2300/1866 ,  H05B6/6408 ,  H05B6/806

    摘要： A micro vial assembly for performing microwave-assisted chemical reactions on small reaction mixture volumes is disclosed, wherein a reaction vessel (10) is sealed through a diaphragm (30) that is capped over an open end of the reaction vessel. The reaction vessel mouths in an end plane of a sleeve (20) surrounding the reaction vessel, the diaphragm being clamped for sealing the open end of the vessel by means of a cap (40) which is secured to the sleeve. The sleeve provides a radial extension of the reaction vessel in order to bridge the radial distance between a wall of the reaction vessel and other components in a system for performing microwave-assisted chemical reactions.

    摘要翻译： 公开了一种用于在小反应混合物体积上进行微波辅助化学反应的微型小瓶组件，其中反应容器（10）通过覆盖在反应容器开口端的隔膜（30）密封。 反应容器在围绕反应容器的套筒（20）的端面中开口，隔膜被夹紧，借助于固定到套筒上的盖（40）密封容器的开口端。 套筒提供反应容器的径向延伸，以便在用于进行微波辅助化学反应的系统中桥接反应容器的壁与其它组分之间的径向距离。

    METHOD OF SEQUENCING DNA
    6.
    发明申请
    METHOD OF SEQUENCING DNA 有权
    序列DNA的方法

    公开(公告)号：US20090155801A1

    公开(公告)日：2009-06-18

    申请号：US12323706

    申请日：2008-11-26

    申请人： Pal Nyren ,  Mostafa Ronaghi ,  Annika Tallsjo

    发明人： Pal Nyren ,  Mostafa Ronaghi ,  Annika Tallsjo

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2565/301

    摘要： The present invention provides a method of identifying a base at a target position in a sample nucleic acid sequence, said method comprising: subjecting a primer hybridised to said sample nucleic acid immediately adjacent to the target position, to a polymerase primer extension reaction in the presence of a nucleotide, whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and determining whether or not said nucleotide is incorporated by detecting whether PPi is released, the identity of the target base being determined from the identity of any nucleotide incorporated, wherein, where said nucleotide comprises an adenine base, an α-thio triphosphate analogue of said nucleotide is used, ant the Rp isomer of said analogue and/or the degradation products of said analogue are eliminated from the polymerase reaction step.

    摘要翻译： 本发明提供了鉴定样品核酸序列中目标位置的碱基的方法，所述方法包括：使与所述目标位置相邻的所述样品核酸杂交的引物在存在的情况下进行聚合酶引物延伸反应 的核苷酸，其中如果核苷酸与目标位置中的碱基互补，则该核苷酸将被引入，并且通过检测PPi是否被释放来确定所述核苷酸是否被并入，靶基因的身份是根据身份确定的 其中，当所述核苷酸包含腺嘌呤碱基时，使用所述核苷酸的α-硫代三磷酸类似物，所述类似物的Rp异构体和/或所述类似物的降解产物的抗体从聚合酶反应步骤中消除 。

    Method of sequencing DNA
    7.
    发明授权
    Method of sequencing DNA 有权
    DNA测序方法

    公开(公告)号：US07459311B2

    公开(公告)日：2008-12-02

    申请号：US10363231

    申请日：2001-09-07

    申请人： Pål Nyren ,  Mostafa Ronaghi ,  Annika Tallsjö

    发明人： Pål Nyren ,  Mostafa Ronaghi ,  Annika Tallsjö

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2565/301

    摘要： The present invention provides a method of identifying a base at a target position in a sample nucleic acid sequence, said method comprising: subjecting a primer hybridised to said sample nucleic acid immediately adjacent to the target position, to a polymerase primer extension reaction in the presence of a nucleotide, whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and determining whether or not said nucleotide is incorporated by detecting whether Ppi is released, the identity of the target base being determined from the identity of any nucleotide incorporated, wherein, where said nucleotide comprises an adenine base, an α-thio triphosphate analogue of said nucleotide is used, and the Rp isomer of said analogue and/or the degradation products of said analogue are eliminated from the polymerase reaction step.

    摘要翻译： 本发明提供了鉴定样品核酸序列中目标位置的碱基的方法，所述方法包括：使与所述目标位置相邻的所述样品核酸杂交的引物在存在的情况下进行聚合酶引物延伸反应 的核苷酸，其核苷酸仅在与目标位置的碱基互补时才被引入，并且通过检测Ppi是否被释放来确定所述核苷酸是否被并入，靶基因的身份是根据身份确定的 其中，当所述核苷酸包含腺嘌呤碱基时，使用所述核苷酸的α-硫代三磷酸类似物，并且所述类似物的Rp异构体和/或所述类似物的降解产物从聚合酶反应步骤中消除 。

    CONDITIONING OF PACKED CHROMATOGRAPHY COLUMNS
    10.
    发明申请
    CONDITIONING OF PACKED CHROMATOGRAPHY COLUMNS 有权

    公开(公告)号：US20210236957A1

    公开(公告)日：2021-08-05

    申请号：US17056587

    申请日：2019-05-21

    申请人： Biotage AB

    发明人： Ioannidis PANAGIOTIS ,  Anders WARNGREN ,  Tobias NORDIN

    IPC分类号： B01D15/16 ,  B01D15/20 ,  G01N30/56

    摘要： The present invention relates to a method of conditioning a dry-packed chromatography column, which method comprises providing a column packed with inert and hydrophilic chromatography resin; and driving a solvent or solvent mix across the column. The solvent(s) are selected to be less hydrophilic than the chromatography resin; and the rate of the solvent flow is controlled to a rate which does not cause the system pressure to exceed a predefined threshold. The present invention is advantageously used in FLASH chromatography, where the generation of heat may pose problems e.g. to plastic frits or filters at high flow rates and/or wetting of dry resins.