公开(公告)号：US4898814A

公开(公告)日：1990-02-06

申请号：US915753

申请日：1986-10-06

申请人： Byoung S. Kwon

发明人： Byoung S. Kwon

IPC分类号： A61K38/00 ,  C12N9/02 ,  C12N15/53

CPC分类号： C12N9/0071 ,  C12Y114/18001 ,  A61K38/00

摘要： This discovery resulted when a .lambda. gt11 cDNA library of normal human melanocytes were screened with antibodies directed against purified hamster tyrosinase. Sixteen independent clones which gave a positive signal were isolated from 5.times.10.sup.5 independent plaques. cDNA inserts of 13 clones among the 16 candidates cross-hybridized with each other, indicating that they were from related mRNA species. mRNA homologous to a representative cDNA .lambda. mel 34 was expressed specifically in melanocytes, detecting an approximately 2.4 kb mRNA species of human melanocytes. The nucleotide sequence of the three overlapping cDNA inserts spanning 1.88 kb was determined and an amino acid sequence was deducted. The human tyrosinase is composed of 548 amino acids with a molecular weight of 62,160 excluding a hydrophobic signal peptide. Mouse genomic DNA blot analysis revealed that the gene for .lambda. mel 34 was deleted in albino mouse homozygous for the deletion at and around the albino locus on chromosome 7. It is concluded that .lambda. mel 34 contained cDNA encoding human tyrosinase. Moreover, the cDNA gene for human tyrosinase was recovered from the .lambda. mel 34 and can be used for many purposes including the production of pure human tyrosinase.

摘要翻译： 当用针对纯化的仓鼠酪氨酸酶的抗体筛选正常人黑素细胞的λgt11cDNA文库时，导致了这一发现。 从5×10 5个独立的斑块中分离得到了16个独立的克隆。 在16个候选物中的13个克隆的cDNA插入物彼此杂交，表明它们来自相关的mRNA种类。 与代表性cDNAλmel 34同源的mRNA在黑素细胞中特异性表达，检测到大约2.4kb的人类黑素细胞的mRNA种类。 确定跨越1.88kb的三个重叠cDNA插入物的核苷酸序列，并扣除氨基酸序列。 人酪氨酸酶由548个氨基酸组成，分子量为62,160，不包括疏水信号肽。 小鼠基因组DNA印迹分析显示，在染色体7上的白化病基因座周围及其周围的缺失中，λmel 34的基因在白化小鼠中是纯合的，其中λmel 34含有编码人酪氨酸酶的cDNA。 此外，从λmel 34回收人酪氨酸酶的cDNA基因，可用于许多目的，包括生产纯人酪氨酸酶。

公开(公告)号：US5196244A

公开(公告)日：1993-03-23

申请号：US614001

申请日：1990-11-13

申请人： William C. Beck

发明人： William C. Beck

IPC分类号： A61F13/00 ,  A61F13/15 ,  A61F13/20

CPC分类号： A61F13/00034 ,  A61F13/2005 ,  A61F13/51478 ,  A61F13/539 ,  A61F2013/00268 ,  A61F2013/0031 ,  A61F2013/00574 ,  A61F2013/00731 ,  A61F2013/00885 ,  A61F2013/00974 ,  A61F2013/15073 ,  A61F2013/15081 ,  A61F2013/51454 ,  A61F2013/53445 ,  Y10S428/913 ,  Y10T428/1334

摘要： The discovery of this invention is that the use of an aseptic barrier in the form of a layer of material which is impervious to the passage of water in combination with at least one layer of absorbent tissue in the form of a disposable tissue or handkerchief can function to reduce materially the transfer of pathogens such as viruses and bacteria from the bodily internal products, mucous and fluid effluent from inside a person to his hand. The above principles may be embodied in a disposable tissue including an aseptic barrier or in a method of entrapping nasal secretions in such a way that contamination of the environment from infected noses be avoided. This is accomplished by the creation of a trap in the form of a mitten-like bag, resistant to the passage of aqueous liquids, with facial tissue affixed to the outside of this mitten-like bag.

摘要翻译： 本发明的发现是使用以一次性组织或手帕形式的与至少一层吸收性组织结合的不透水的材料的材料层的形式的无菌屏障可以起作用 从身体内部产品，人体内部的粘液和流体流出物等手段，大大减少病毒和细菌等病原体的转移。 上述原理可以体现在包括无菌屏障的一次性组织中，或以如下方式捕获鼻分泌物的方法：避免环境受感染的鼻子的污染。 这是通过产生一种手套形式的捕获器来实现的，该捕获器抵抗含水液体的通过，并且将面巾粘贴在该手套状袋子的外部。

公开(公告)号：US5679511A

公开(公告)日：1997-10-21

申请号：US891942

申请日：1992-06-01

申请人： Byoung Se Kwon

发明人： Byoung Se Kwon

IPC分类号： A61K38/00 ,  C12N9/02 ,  C12Q1/68 ,  C07K14/00 ,  C12N15/11 ,  C12N15/70

CPC分类号： C12N9/0071 ,  A61K38/00

摘要： The present invention started when a .lambda.gt11 cDNA library of normal human melanocytes was screened with antibodies directed against purified hamster tyrosinase. Two important cDNA clones were isolated: cDNA .lambda.mel 34 and .lambda.mel 17-1. It is concluded that .lambda.mel 34 contained cDNA encoding human tyrosinase. Moreover, the cDNA gene for human tyrosinase was recovered from the .lambda.mel 34 and can be used for many purposes including the production of pure human tyrosinase. This gene and its promoter are characterized herein. Studies suggested that .lambda.mel 17-1 gene product act on melanin biosynthesis's pathway distal to tyrosinase. Melanocytes preferentially express an mRNA species, Pmel 17, whose protein product cross reacted with anti-tyrosinase antibodies and whose expression correlated with the melanin content. The deduced protein structure has been analyzed and its chromosomal location in mouse and man has been mapped. The human Pmel 17 gene, designated D12S53E, maps to Chromosome 12, region 12pter-q21; and the mouse homologue, designated D12S53Eh, maps to the distal region of mouse Chromosome 10, a region also known to carry the coat color locus si (silver).

摘要翻译： 当用针对纯化的仓鼠酪氨酸酶的抗体筛选正常人黑素细胞的λgt11cDNA文库时，开始本发明。 分离出两个重要的cDNA克隆：cDNAλmel 34和λmel 17-1。 结论是λmel 34含有编码人酪氨酸酶的cDNA。 此外，从λmel 34回收人酪氨酸酶的cDNA基因，可用于许多目的，包括生产纯人酪氨酸酶。 该基因及其启动子在本文中被表征。 研究表明，λmel 17-1基因产物作用于黑色素生物合成途径远离酪氨酸酶。 黑素细胞优先表达mRNA，Pmel 17，其蛋白质产物与抗酪氨酸酶抗体交叉反应，其表达与黑色素含量相关。 已经分析了推导的蛋白质结构，并对其在小鼠和人中的染色体位置进行了定位。 命名为D12S53E的人类Pmel 17基因映射到染色体12，区域12pter-q21; 并且称为D12S53Eh的小鼠同源物映射到小鼠染色体10的远端区域，该区域也称为携带着色轨迹si（银）的区域。