公开(公告)号：US20070178558A1

公开(公告)日：2007-08-02

申请号：US11727730

申请日：2007-03-28

申请人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

发明人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12N9/02 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/0073 ,  C12Y114/13016

摘要： Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.

摘要翻译： 来自Comamonas（以前为假单胞菌）的环戊酮1,2-单加氧酶（CPMO） 菌株NCIMB 9872进行降解途径的第二步，其允许细菌使用环戊醇作为生长的唯一碳源。 在本发明中，报道了CPMO编码基因（cpnB）在4.3kb SphI片段上的定位，确定其序列。 发现该基因编码的550个氨基酸的CPMO多肽（M >r，62,111）与不动杆菌属的环己酮1,2-单加氧酶（CHMO）的序列具有36.5％的同一性。 菌株NCIMB 9871.62-kDa CPMO在大肠杆菌中表达为IPTG诱导蛋白。

公开(公告)号：US4929011A

公开(公告)日：1990-05-29

申请号：US338156

申请日：1989-04-14

申请人： Ron Vandersluis ,  Erik Quittner

发明人： Ron Vandersluis ,  Erik Quittner

IPC分类号： B25J15/02 ,  B64G1/64

CPC分类号： B25J15/0206 ,  B64G1/646

摘要： The grapple fixture, with improved docking and engaging capabilities, is provided for use with, for example, a relocatable space station remote manipulator system. The grapple fixture is for use with an end effector having grappling capabilities and latching capabilities and which may include a force moment sensor which can cooperate with the arm in order to align the end effector with the grapple fixture without the aid of a grappling mechanism. The grapple fixture comprises a base, having a flange and a cylinder with an end effector abutment face, a plurality of end effector latching trunnions extending radially from the cylinder, a cone-shaped, grapple fixture aligning body with a cylindrical, end effector locating base portion attached to the abutment face, and a plurality of laterally extending, grapple fixture recess engaging, rounded shoulders spaced around the cylindrical base.

摘要翻译： 提供了具有改进的对接和接合能力的抓斗夹具，用于例如可重新定位的空间站远程操纵器系统。 抓斗夹具用于具有抓握能力和闩锁能力的末端执行器，并且其可以包括力矩传感器，该力矩传感器可以与臂配合，以便在没有抓斗机构的帮助下将末端执行器与抓斗夹具对准。 抓斗夹具包括底座，具有凸缘和具有端部执行器接合面的圆柱体，从圆柱体径向延伸的多个端部执行器闩锁耳轴，锥形的抓斗固定器本体，其具有圆柱形的末端执行器定位基座 附接到邻接面的部分，以及多个横向延伸的抓斗固定凹部，其接合围绕圆柱形基座间隔开的圆形肩部。