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18 条记录 (耗时 0.014 秒)

    Gene encoding cyclododecanone monooxygenase
    1.
    发明申请
    Gene encoding cyclododecanone monooxygenase 失效
    基因编码环十二酮单加氧酶

    公开(公告)号：US20070184531A1

    公开(公告)日：2007-08-09

    申请号：US11717247

    申请日：2007-03-13

    申请人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

    发明人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/02 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/0073 ,  C12N1/20 ,  C12P17/08 ,  C12R1/40

    摘要： The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.

    摘要翻译： 本发明涉及恶臭假单胞菌（称为HI-70）的新菌株，并且从所述菌株中分离，克隆和测序来自所述菌株的环十二酮单加氧酶编码基因（命名为cdnB）。 本发明还涉及新的环十二酮单加氧酶和使用环十二酮单加氧酶编码基因的方法。

    Cloning, sequencing and expression of a Comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli
    2.
    发明申请
    Cloning, sequencing and expression of a Comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli 失效
    在大肠杆菌中克隆，测序和表达一个Comamonas环戊酮1,2-单加氧酶编码基因

    公开(公告)号：US20070178558A1

    公开(公告)日：2007-08-02

    申请号：US11727730

    申请日：2007-03-28

    申请人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

    发明人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

    IPC分类号： C07H21/04 ,  C12P21/06 ,  C12N9/02 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/0073 ,  C12Y114/13016

    摘要： Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.

    摘要翻译： 来自Comamonas（以前为假单胞菌）的环戊酮1,2-单加氧酶（CPMO） 菌株NCIMB 9872进行降解途径的第二步，其允许细菌使用环戊醇作为生长的唯一碳源。 在本发明中，报道了CPMO编码基因（cpnB）在4.3kb SphI片段上的定位，确定其序列。 发现该基因编码的550个氨基酸的CPMO多肽（M >r，62,111）与不动杆菌属的环己酮1,2-单加氧酶（CHMO）的序列具有36.5％的同一性。 菌株NCIMB 9871.62-kDa CPMO在大肠杆菌中表达为IPTG诱导蛋白。

    Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli
    3.
    发明申请
    Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli 失效
    在大肠杆菌中克隆，测序和表达一个环戊烯酮-1,2-单加氧酶编码基因

    公开(公告)号：US20050089846A1

    公开(公告)日：2005-04-28

    申请号：US10312585

    申请日：2001-07-13

    申请人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

    发明人： Hiroaki Iwaki ,  Yoshie Hasegawa ,  Peter Lau

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/53 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N9/0073 ,  C12Y114/13016

    摘要： Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mt, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.

    摘要翻译： 来自Comamonas（以前为假单胞菌）的环戊酮1,2-单加氧酶（CPMO） 菌株NCIMB 9872进行降解途径的第二步，其允许细菌使用环戊醇作为生长的唯一碳源。 在本发明中，报道了CPMO编码基因（cpnB）在4.3kb SphI片段上的定位，确定其序列。 发现由该基因编码的550个氨基酸的CPMO多肽（MATT，62,111）与不动杆菌属的环己酮1,2-单加氧酶（CHMO）的序列具有36.5％的同一性。 菌株NCIMB 9871.62-kDa CPMO在大肠杆菌中表达为IPTG诱导蛋白。

    Method and device for the insertion of cans into carriers
    4.
    发明授权
    Method and device for the insertion of cans into carriers 失效
    用于将罐插入载体的方法和装置

    公开(公告)号：US4240239A

    公开(公告)日：1980-12-23

    申请号：US959936

    申请日：1978-11-13

    申请人： Herbert A. Stelzner ,  Peter Lau

    发明人： Herbert A. Stelzner ,  Peter Lau

    IPC分类号： B65B17/02 ,  B65B21/00

    CPC分类号： B65B17/025

    摘要： A method and apparatus for packaging cans or similar objects having beaded rims in combination with planar carriers of polymeric materials is described. The cans are arrayed into groups corresponding to rim-engagingopenings or formations on the carriers by a conveyor and the cans are then positioned, aligned and firmly held with respect to the formations by cores inserted into the interstices within the array. The cores are actuated by the position of the conveyor belt via rails at specific stations. While the cores are holding the cans in alignment with respect to the carrier, the cans are inserted into the carriers by conveyor-actuated rams so that the beads on said rims engage the formations on the carriers. The carriers may include the rim-engaging formations along opposite faces.

    摘要翻译： 描述了一种用于将具有串珠边缘与聚合材料的平面载体组合的罐或类似物体的方法和装置。 罐通过传送器排列成对应于载体上的轮辋接合开口或结构的组，然后通过插入到阵列内的间隙中的芯相对于结构将罐定位，对准和牢固地保持罐。 通过在特定车站的轨道通过传送带的位置来驱动磁芯。 当芯部相对于载体保持罐对准时，罐通过输送机致动的公柱插入载体中，使得所述轮辋上的小珠接合载体上的结构。 载体可以包括沿相对面的轮辋接合结构。

    Forward baseband digitalization
    9.
    发明授权
    Forward baseband digitalization 有权
    正向基带数字化

    公开(公告)号：US07706689B2

    公开(公告)日：2010-04-27

    申请号：US11150908

    申请日：2005-06-13

    申请人： Oleh Sniezko ,  Krzysztof Pradzynski ,  Guy Sucharczuk ,  Peter Lau ,  Charles Barker

    发明人： Oleh Sniezko ,  Krzysztof Pradzynski ,  Guy Sucharczuk ,  Peter Lau ,  Charles Barker

    IPC分类号： H04J14/02

    CPC分类号： H04J14/0298 ,  H04B10/25751 ,  H04B10/29 ,  H04J14/00 ,  H04J14/02

    摘要： Methods and apparatus are described for forward baseband digitalization. A method includes receiving a forward baseband digital optical signal from an optical fiber; transforming the forward baseband digital optical signal to a forward analog electrical signal; transmitting the forward analog electrical signal on an electrical conductor; receiving a reverse analog electrical signal on the electrical conductor; transforming the reverse analog electrical signal to a reverse digital baseband optical signal; and transmitting the reverse digital baseband optical signal. An apparatus includes a forward baseband digital optical signal receiver; a forward circuit coupled to the forward baseband digital optical signal receiver, the forward circuit transforming a forward baseband digital optical signal to a forward analog electrical signal; a forward analog electrical signal transmitter coupled to the forward circuit; a reverse analog electrical signal receiver; a reverse circuit coupled to the reverse analog electrical signal receiver, the reverse circuit transforming a reverse analog electrical signal to a reverse digital baseband optical signal; and a reverse baseband digital transmitter coupled to the reverse circuit.

    摘要翻译： 描述了用于正向基带数字化的方法和装置。 一种方法包括从光纤接收正向基带数字光信号; 将正向基带数字光信号转换成正向模拟电信号; 在电导体上传送正向模拟电信号; 在电导体上接收反向模拟电信号; 将反向模拟电信号转换成反向数字基带光信号; 并发送反向数字基带光信号。 一种装置包括：正向基带数字光信号接收机; 正向电路耦合到正向基带数字光信号接收器，正向电路将正向基带数字光信号变换为正向模拟电信号; 耦合到正向电路的正向模拟电信号发射器; 反向模拟电信号接收器; 反向电路，耦合到反向模拟电信号接收器，反向电路将反向模拟电信号转换成反向数字基带光信号; 以及耦合到反向电路的反向基带数字发射机。

    Forward baseband digitalization
    10.
    发明申请
    Forward baseband digitalization 有权
    正向基带数字化

    公开(公告)号：US20050286556A1

    公开(公告)日：2005-12-29

    申请号：US11150908

    申请日：2005-06-13

    申请人： Oleh Sniezko ,  Krzysztof Pradzynski ,  Guy Sucharczuk ,  Peter Lau ,  Charles Barker

    发明人： Oleh Sniezko ,  Krzysztof Pradzynski ,  Guy Sucharczuk ,  Peter Lau ,  Charles Barker

    IPC分类号： H04B10/272 ,  H04B10/29 ,  H04J3/16

    CPC分类号： H04J14/0298 ,  H04B10/25751 ,  H04B10/29 ,  H04J14/00 ,  H04J14/02

    摘要： Methods and apparatus are described for forward baseband digitalization. A method includes receiving a forward baseband digital optical signal from an optical fiber; transforming the forward baseband digital optical signal to a forward analog electrical signal; transmitting the forward analog electrical signal on an electrical conductor; receiving a reverse analog electrical signal on the electrical conductor; transforming the reverse analog electrical signal to a reverse digital baseband optical signal; and transmitting the reverse digital baseband optical signal. An apparatus includes a forward baseband digital optical signal receiver; a forward circuit coupled to the forward baseband digital optical signal receiver, the forward circuit transforming a forward baseband digital optical signal to a forward analog electrical signal; a forward analog electrical signal transmitter coupled to the forward circuit; a reverse analog electrical signal receiver; a reverse circuit coupled to the reverse analog electrical signal receiver, the reverse circuit transforming a reverse analog electrical signal to a reverse digital baseband optical signal; and a reverse baseband digital transmitter coupled to the reverse circuit.

    摘要翻译： 描述了用于正向基带数字化的方法和装置。 一种方法包括从光纤接收正向基带数字光信号; 将正向基带数字光信号转换成正向模拟电信号; 在电导体上传输正向模拟电信号; 在电导体上接收反向模拟电信号; 将反向模拟电信号转换成反向数字基带光信号; 并发送反向数字基带光信号。 一种装置包括：正向基带数字光信号接收机; 正向电路耦合到正向基带数字光信号接收器，正向电路将正向基带数字光信号变换为正向模拟电信号; 耦合到正向电路的正向模拟电信号发射器; 反向模拟电信号接收机; 反向电路，耦合到反向模拟电信号接收器，反向电路将反向模拟电信号转换成反向数字基带光信号; 以及耦合到反向电路的反向基带数字发射机。