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    SSB METHOD
    1.
    发明申请
    SSB METHOD 有权

    公开(公告)号：US20220145383A1

    公开(公告)日：2022-05-12

    申请号：US17481374

    申请日：2021-09-22

    Applicant: Oxford Nanopore Technologies Limited

    Inventor： James White ,  Ruth Moysey ,  Mihaela Misca

    IPC: C12Q1/6869

    Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.

    SSB method
    4.
    发明授权
    SSB method 有权

    公开(公告)号：US11155860B2

    公开(公告)日：2021-10-26

    申请号：US14415459

    申请日：2013-07-18

    Applicant: Oxford Nanopore Technologies Limited

    Inventor： James White ,  Ruth Moysey ,  Mihaela Misca

    IPC: C12Q1/6869

    Abstract: The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.

    METHOD
    8.
    发明申请
    METHOD 有权

    公开(公告)号：US20210198732A1

    公开(公告)日：2021-07-01

    申请号：US17057863

    申请日：2019-05-24

    Applicant: Oxford Nanopore Technologies Limited

    Inventor： James Edward Graham ,  Etienne Raimondeau ,  Rebecca Victoria Bowen

    IPC: C12Q1/6869 ,  C12Q1/6806

    Abstract: A method for selectively modifying a target polynucleotide in a sample of polynucleotides, the method comprising contacting a sample of polynucleotides with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein such that the polynucleotide-guided effector protein cuts the target polynucleotide to produce a cut end comprising an overhang; and attaching an adapter to the cut end in the target polynucleotide.

    METHOD OF DETERMINING THE PRESENCE OR ABSENCE OF A TARGET ANALYTE COMPRISING USING A REPORTER POLYNUCLEOTIDE AND A TRANSMEMBRANE PORE
    10.
    发明申请
    METHOD OF DETERMINING THE PRESENCE OR ABSENCE OF A TARGET ANALYTE COMPRISING USING A REPORTER POLYNUCLEOTIDE AND A TRANSMEMBRANE PORE 有权

    公开(公告)号：US20210148903A1

    公开(公告)日：2021-05-20

    申请号：US16610891

    申请日：2018-05-03

    Applicant: Oxford Nanopore Technologies Limited

    Inventor： Daniel George Fordham

    IPC: G01N33/543

    Abstract: The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.

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