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    Analysis of a polynucleotide via a nanopore system
    6.
    发明授权
    Analysis of a polynucleotide via a nanopore system 有权

    公开(公告)号：US11085077B2

    公开(公告)日：2021-08-10

    申请号：US16162848

    申请日：2018-10-17

    申请人： Oxford Nanopore Technologies Limited

    发明人： Stuart William Reid ,  Gavin Harper

    IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6806

    摘要： A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide. The predetermined sequence of the clock nucleotides comprises a restriction site for a restriction enzyme and at least one further nucleotide that extends the predetermined sequence.

    APPARATUS AND METHODS FOR CONTROLLING INSERTION OF A MEMBRANE CHANNEL INTO A MEMBRANE
    9.
    发明申请
    APPARATUS AND METHODS FOR CONTROLLING INSERTION OF A MEMBRANE CHANNEL INTO A MEMBRANE 审中-公开

    公开(公告)号：US20200179920A1

    公开(公告)日：2020-06-11

    申请号：US16463617

    申请日：2017-11-24

    申请人： Oxford Nanopore Technologies Limited

    发明人： Daniel Garalde ,  James Clarke ,  Mike Jennison ,  Andrew Heron

    IPC分类号： B01L3/00 ,  B81C1/00

    摘要： Apparatus and methods for controlling the insertion of a membrane channel into a membrane are disclosed. In one arrangement a first bath holds a first liquid in contact with a first surface of a membrane. A second bath holds a second liquid in contact with a second surface of the membrane. The membrane separates the first and second liquids. A first electrode contacts the first liquid. A second electrode contacts the second liquid. A driving unit applies a potential difference across the membrane via the first and second electrodes to promote insertion of a membrane channel into the membrane from the first liquid or the second liquid. A membrane voltage reduction unit is connected in series with the membrane. The driving unit applies a driving voltage across the membrane voltage reduction unit and the membrane, the driving voltage providing the potential difference across the membrane. The membrane voltage reduction unit is configured such that a reduction in resistance through the membrane caused by insertion of a membrane channel intrinsically increases a potential difference across the membrane voltage reduction unit thereby lowering the potential difference across the membrane. The lowering of the potential difference across the membrane is sufficient to prevent or reduce promotion of insertion of a further membrane channel.

    One-way valve
    10.
    发明授权
    One-way valve 有权

    公开(公告)号：US10054234B2

    公开(公告)日：2018-08-21

    申请号：US14232477

    申请日：2012-07-11

    申请人： Anthony Jones ,  Jonathan Upsdell

    发明人： Anthony Jones ,  Jonathan Upsdell

    IPC分类号： F16K7/17 ,  F16K25/00 ,  F16K15/14

    CPC分类号： F16K7/17 ,  F16K15/144 ,  F16K25/00 ,  Y10T137/0318 ,  Y10T137/7895

    摘要： A one-way valve, comprises a valve housing; a valve member provided within the valve housing, the valve member being operative to open and close the valve and comprising a diaphragm with a central orifice to allow fluid to pass from one side of the diaphragm to the other; a valve inlet provided on a first side of the diaphragm; and a valve outlet provided on a second side of the diaphragm; wherein the configuration of the diaphragm and the valve housing biases the diaphragm to seal the inlet at rest.