公开(公告)号：US4826760A

公开(公告)日：1989-05-02

申请号：US922243

申请日：1986-10-23

申请人： James R. Privitera

发明人： James R. Privitera

IPC分类号： G01N33/569 ,  G01N33/546 ,  G01N33/547 ,  G01N33/577

CPC分类号： G01N33/56972 ,  Y10T436/25375

摘要： An improved method for determining a helper/suppressor ratio of lysed whole blood. The process includes the steps of high speed microcentrifuging the lysed whole blood, decanting, washing and resuspending the sample followed by a second high speed microcentrifuging step. The supernatant fluid is poured off and a tagging solution containing colored beads which bind to T4 and T8 lymphocytes is added followed by low speed microcentrifuging, incubating and counting under a microscope.

摘要翻译： 一种用于确定裂解全血的辅助/抑制比的改进方法。 该方法包括以下步骤：将裂解的全血高速微量离心，倾析，洗涤和重新悬浮样品，然后进行第二次高速微量离心步骤。 倒出上清液，加入含有与T4和T8淋巴细胞结合的着色珠粒的标记溶液，然后低速微量离心，在显微镜下孵育和计数。