公开(公告)号：US5733778A

公开(公告)日：1998-03-31

申请号：US452531

申请日：1995-05-30

申请人： Gregory Matlashewski ,  Hugues Chareat

发明人： Gregory Matlashewski ,  Hugues Chareat

IPC分类号： C12N15/09 ,  A61K35/68 ,  A61K39/00 ,  A61K39/008 ,  A61K39/395 ,  A61P33/02 ,  C07K14/44 ,  C07K16/20 ,  C12N1/10 ,  C12N15/30 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  C12R1/90 ,  H01S3/02 ,  C12N15/63 ,  C12N15/12

CPC分类号： A61K39/008 ,  A61K35/68 ,  C07K14/44 ,  C07K16/20 ,  A61K2039/515 ,  A61K39/00 ,  Y10S530/82 ,  Y10S530/822

摘要： Differentially expressed Leishmania genes and proteins are described. One differentially expressed gene (A2) is expressed at significantly elevated levels (more than about 10 fold higher) in the amastigote stage of the life cycle when the Leishmania organism is present in macrophages than in the free promastigote stage. The A2 gene encodes a 22 kD protein (A2 protein) that is recognized by kala-azar convalescent serum and has amino acid sequence homology with an S-antigen of Plasmodium falciparum Vietnamese isolate VI. Differentially expressed Leishmania genes and proteins have utility as vaccines, diagnostic reagents, as tools for the generation of immunological reagents and the generation of attenuated variants of Leishmania.

摘要翻译： 描述了差异表达的利什曼原虫基因和蛋白质。 当利什曼原虫生物体存在于巨噬细胞中时，一个差异表达的基因（A2）在生命周期的无性系反应阶段以显着提高的水平（超过约10倍）表达，而不是在游离前列腺素阶段。 A2基因编码由kala-azar恢复期血清识别并与恶性疟原虫越南分离株VI的S-抗原具有氨基酸序列同源性的22kD蛋白（A2蛋白）。 差异表达的利什曼原虫基因和蛋白质可用作疫苗，诊断试剂，作为产生免疫试剂和产生减毒变种的利什曼原虫的工具。

公开(公告)号：US5485841A

公开(公告)日：1996-01-23

申请号：US388302

申请日：1995-02-14

申请人： Kenneth L. Watkin ,  Peter T. Macklem

发明人： Kenneth L. Watkin ,  Peter T. Macklem

IPC分类号： A61B8/08 ,  G01S7/52 ,  A61B8/00

CPC分类号： A61B8/08 ,  G01S7/52036

摘要： A diagnostic method and apparatus for assessing lung tissue during breathing, transmits ultrasonic radiation into the lung tissue; reflected or echo radiation, is reflected dynamically by air spaces in the tissue; a signal is developed from the reflected or echo radiation as a dynamic real-time measure of parameters of the air spaces, particularly size and density or number, indicative of the state of the lung tissue.

摘要翻译： 呼吸时评估肺组织的诊断方法和装置，将超声波辐射传输到肺组织; 反射或回波辐射，被组织中的空气空间动态反射; 作为用于指示肺组织的状态的空气空间，特别是大小和密度或数量的参数的动态实时测量，从反射或回波辐射开发信号。

公开(公告)号：US5780591A

公开(公告)日：1998-07-14

申请号：US460746

申请日：1995-06-02

申请人： Gregory Matlashewski ,  Hugues Charest

发明人： Gregory Matlashewski ,  Hugues Charest

IPC分类号： C12N15/09 ,  A61K35/68 ,  A61K39/00 ,  A61K39/008 ,  A61K39/395 ,  A61P33/02 ,  C07K14/44 ,  C07K16/20 ,  C12N1/10 ,  C12N15/30 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  C12R1/90 ,  H01S3/02

CPC分类号： A61K39/008 ,  A61K35/68 ,  C07K14/44 ,  C07K16/20 ,  A61K2039/515 ,  A61K39/00 ,  Y10S530/82 ,  Y10S530/822

摘要： Differentially expressed Leishmania genes and proteins are described. One differentially expressed gene (A2) is expressed at significantly elevated levels (more than about 10 fold higher) in the amastigote stage of the life cycle when the Leishmania organism is present in macrophages than in the free promastigote stage. The A2 gene encodes a 22 kD protein (A2 protein) that is recognized by kala-azar convalescent serum and has amino acid sequence homology with an S-antigen of Plasmodium falciparum Vietnamese isolate VI. Differentially expressed Leishmania genes and proteins have utility as vaccines, diagnostic reagents, as tools for the generation of immunological reagents and the generation of attenuated variants of Leishmania.

摘要翻译： 描述了差异表达的利什曼原虫基因和蛋白质。 当利什曼原虫生物体存在于巨噬细胞中时，一个差异表达的基因（A2）在生命周期的无性系反应阶段以显着提高的水平（超过约10倍）表达，而不是在游离前列腺素阶段。 A2基因编码由kala-azar恢复期血清识别并与恶性疟原虫越南分离株VI的S-抗原具有氨基酸序列同源性的22kD蛋白（A2蛋白）。 差异表达的利什曼原虫基因和蛋白质可用作疫苗，诊断试剂，作为产生免疫试剂和产生减毒变种的利什曼原虫的工具。