公开(公告)号：US08247535B2

公开(公告)日：2012-08-21

申请号：US13101351

申请日：2011-05-05

申请人： Volker Heinrichs ,  Laura Cooper Schouten ,  Brian Vande Berg

发明人： Volker Heinrichs ,  Laura Cooper Schouten ,  Brian Vande Berg

IPC分类号： C12N15/88 ,  A61K38/43 ,  A61K380/00

CPC分类号： C12N15/8275 ,  C12N9/1092

摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.

摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。