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    Human lysozyme gene, its encoding polypeptide and the method preparing for them
    2.
    发明授权
    Human lysozyme gene, its encoding polypeptide and the method preparing for them 有权
    人溶菌酶基因，其编码多肽及其制备方法

    公开(公告)号：US06436688B1

    公开(公告)日：2002-08-20

    申请号：US09786023

    申请日：2001-06-21

    申请人： Long Yu ,  Qiang Fu ,  Yong Zhao ,  Honglai Zhang ,  Anding Bi

    发明人： Long Yu ,  Qiang Fu ,  Yong Zhao ,  Honglai Zhang ,  Anding Bi

    IPC分类号： C12N936

    CPC分类号： C12N9/2462

    摘要： The invention relates to a novel member LYC2 of lysozyme gene family. The invention provides the cDNA sequence encoding for the novel lysozyme, the polypeptide encoded by the sequence, as well as the method for producing said novel human lysozyme utilizing recombinant technology. The invention also provides the use of the novel human lysozyme.

    摘要翻译： 本发明涉及溶菌酶基因家族的新型成员LYC2。 本发明提供编码新溶菌酶的cDNA序列，由序列编码的多肽，以及利用重组技术生产所述新型人溶菌酶的方法。 本发明还提供了新型人溶菌酶的用途。

    Human lysozyme gene, it's encoded polypeptide and the method of preparing them
    3.
    发明授权
    Human lysozyme gene, it's encoded polypeptide and the method of preparing them 有权
    人溶菌酶基因，其编码多肽及其制备方法

    公开(公告)号：US06660512B1

    公开(公告)日：2003-12-09

    申请号：US09786025

    申请日：2001-06-08

    申请人： Long Yu ,  Qiang Fu ,  Yong Zhao ,  Honglai Zhang ,  Anding Bi

    发明人： Long Yu ,  Qiang Fu ,  Yong Zhao ,  Honglai Zhang ,  Anding Bi

    IPC分类号： C12N936

    CPC分类号： C12N9/2462

    摘要： The invention relates to LYC4, a novel member of the lysozyme gene family. The invention provides the cDNA sequence encoding the novel lysozyme, the polypeptide encoded by the sequence, as well as methods for producing said novel human lysozyme utilizing recombinant technology. The invention also provides the use of the novel human lysozyme.

    摘要翻译： 本发明涉及溶菌酶基因家族的新成员LYC4。 本发明提供了编码新型溶菌酶的cDNA序列，序列编码的多肽以及利用重组技术生产所述新型人溶菌酶的方法。 本发明还提供了新型人溶菌酶的用途。

    Lysozyme dimer
    4.
    发明授权
    Lysozyme dimer 失效
    溶菌酶二聚体

    公开(公告)号：US06316236B1

    公开(公告)日：2001-11-13

    申请号：US09130810

    申请日：1998-08-07

    申请人： Peter Herrmann ,  Peter Klein

    发明人： Peter Herrmann ,  Peter Klein

    IPC分类号： C12N936

    CPC分类号： C12N9/2462

    摘要： A method is provided for preparing a highly purified lysozyme dimer product which comprises dimerizing the lysozyme monomer with a suberimidate coupling reagent in a buffer solution adjusted to pH 10, stopping the dimerization at a given point by lowering the pH to 7 via addition of HCl or other suitable acid solution, and purifying the dimeric lysozyme by a series of elution steps carried out using ion exchange resin column chromatography. Monomeric lysozyme remaining undimerized by the initial dimerization step is recycled into the process in order to increase yield. The present system is advantageous in that large amounts of purified lysozyme dimer can be prepared efficiently and relatively inexpensively, and the lysozyme dimer so produced is useful in treating viral and/or bacterial diseases without causing the cytotoxic effects associated with the lysozyme monomer.

    摘要翻译： 提供了一种制备高纯度溶菌酶二聚体产物的方法，其包括将溶菌酶单体与缓冲溶液中的苏木精偶联试剂二聚化，调节至pH为10，通过加入HCl将pH降至7，停止给定点的二聚 其他合适的酸溶液，并通过使用离子交换树脂柱色谱法进行的一系列洗脱步骤纯化二聚溶菌酶。 通过初始二聚步骤保持未稳定化的单体溶菌酶被再循环到该方法中以提高产率。 本发明的系统的优点在于可以有效和相对廉价地制备大量纯化的溶菌酶二聚体，所产生的溶菌酶二聚体可用于治疗病毒和/或细菌疾病，而不会引起与溶菌酶单体相关的细胞毒性作用。