公开(公告)号：WO2022216824A1

公开(公告)日：2022-10-13

申请号：PCT/US2022/023674

申请日：2022-04-06

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： MILLER, Peter J. ,  KENNEDY-DARLING, Julia

IPC: C12Q1/6841

Abstract: Methods for determining locations of analytes include: (a) exposing a biological sample to a plurality of different types of probes, where each different type of probe includes a nucleic acid capture moiety and a detection moiety that includes at least one reporter moiety, where the at least one reporter moiety features multiple label regions, each of the label regions including an oligonucleotide having a sequence; (b) exposing the biological sample to a plurality of optical labels; (c) measuring optical signals generated by optical labels; (d) repeating steps (b) and (c) with different pluralities of optical labels; (e) identifying one or more of the reporter moieties in the sample based on the measured optical signals; and (f) determining a location of one or more of the RNA analytes in the sample based on the identified reporter moieties.

公开(公告)号：WO2020163397A3

公开(公告)日：2020-08-13

申请号：PCT/US2020/016667

申请日：2020-02-04

Applicant: AKOYA BIOSCIENCES, INC. ,  DAKSHINAMOORTHY, Gajalakshmi

Inventor： DAKSHINAMOORTHY, Gajalakshmi ,  MILLER, Peter ,  KENNEDY-DARLING, Julia ,  ZHENG, Yi ,  HOYT, Clifford C.

IPC: C12Q1/6804 ,  C12Q1/6813 ,  G01N33/58

Abstract: The disclosure features methods that include: contacting a biological sample having a first target analyte with a first agent, where the first agent includes a first binding species that specifically binds to the first target analyte, and a first oligonucleotide conjugated to the binding species; contacting the biological sample with a second agent, where the second agent includes a first reactive species and a second oligonucleotide conjugated to the first reactive species, to hybridize at least a portion of the second oligonucleotide to at least a portion of the first oligonucleotide; and contacting the biological sample with a first labeling species, where the first labeling species reacts with the first reactive species to deposit the first labeling species or a derivative thereof in the biological sample.

公开(公告)号：WO2022020340A1

公开(公告)日：2022-01-27

申请号：PCT/US2021/042353

申请日：2021-07-20

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： MCLANE, Michael

IPC: G01N21/64 ,  G01N33/53 ,  G01N33/58

Abstract: Methods include applying a first stain composition comprising a fluorescent counterstain to a biological sample, measuring information corresponding to one or more stains of the first stain composition, removing the fluorescent counterstain from the biological sample, applying a second stain composition to the biological sample, measuring information corresponding to one or more stains of the second stain composition in the biological sample, where the one or more stains include a fluorescent label, and generating a first image of the biological sample, where the first image corresponds to a pattern of simulated hematoxylin staining in the biological sample.

公开(公告)号：WO2022241227A1

公开(公告)日：2022-11-17

申请号：PCT/US2022/029212

申请日：2022-05-13

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： NOLAN, Garry ,  KENNEDY-DARLING, Julia ,  MILLER, Peter J.

IPC: C12Q1/682 ,  C12Q1/6818

Abstract: Methods include contacting a biological sample with a first probe, where the first probe includes a capture moiety having an oligonucleotide sequence that selectively binds to a RNA in the sample, and a secondary oligonucleotide region that does not bind to the RNA, contacting the sample with a second probe, where the second probe includes a probe binding region that is complementary to, and hybridizes to, a portion of the secondary oligonucleotide region, and includes a reporter moiety, and extending the secondary oligonucleotide region using the second probe as a template to generate an extended secondary oligonucleotide region featuring multiple copies of the reporter moiety, where the reporter moiety includes a plurality of label regions each featuring an oligonucleotide sequence, and one or more of the label regions of the reporter moiety are different from the other label regions of the reporter moiety.

公开(公告)号：WO2021067475A1

公开(公告)日：2021-04-08

申请号：PCT/US2020/053600

申请日：2020-09-30

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： CARLSON, Grady

IPC: C12Q1/6804 ,  C12Q1/6841 ,  G01N33/535

Abstract: Methods for imaging an analyte in a sample include contacting the biological sample with a binding agent, where the binding agent includes a binding moiety that binds to the analyte and a first nucleotide sequence, contacting the biological sample with a catalytic agent, where the catalytic agent includes a second nucleotide sequence linked to an enzyme, and where the second nucleotide sequence hybridizes to the first nucleotide sequence, contacting the biological sample with a localization agent, where the localization agent includes a substrate complementary to the enzyme and a third nucleotide sequence linked to the substrate, and contacting the biological sample with a labeling agent, where the labeling agent includes a fourth nucleotide sequence linked to an optical label, where the fourth nucleotide sequence hybridizes to the third nucleotide sequence.

公开(公告)号：WO2020092835A1

公开(公告)日：2020-05-07

申请号：PCT/US2019/059255

申请日：2019-10-31

Applicant: AKOYA BIOSCIENCES, INC. ,  DAKSHINAMOORTHY, Gajalakshmi

Inventor： DAKSHINAMOORTHY, Gajalakshmi ,  KENNEDY-DARLING, Julia

IPC: A61K39/00 ,  A61K47/48 ,  C07K16/00 ,  C12N5/00 ,  C12N9/96 ,  C12P19/34 ,  C12Q1/68

Abstract: The tissue microenvironment is a critical factor to disease mechanism and therapeutic efficiency. Provided here is an imaging platform, methods, and kits which enable measurement of tens of parameters simultaneously within a single tissue, with the potential to reveal unique and important biological associations related to the spatial dimension. The sensitivity of the platform, methods, and kits described herein extends to measure low level markers which can be important for tracking disease progression, diagnosing disease, or both.

公开(公告)号：WO2023014825A1

公开(公告)日：2023-02-09

申请号：PCT/US2022/039323

申请日：2022-08-03

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： MILLER, Peter, J. ,  KENNEDY-DARLING, Julia

IPC: C12Q1/6841 ,  C12Q1/682

Abstract: Methods for labeling biological samples include (a) contacting a biological sample featuring a target RNA with a probe, where the probe includes a capture moiety that specifically binds to the target RNA, and a plurality of reporter moieties, and (b) for each reporter moiety of the plurality of reporter moieties: contacting the reporter moiety with a catalytic agent that includes an oligonucleotide that specifically binds to the reporter moiety, and a reactive species linked to the oligonucleotide, and contacting the biological sample with a labeling agent that reacts with the reactive species to deposit the labeling agent or a derivative thereof in the sample in proximity to the target RNA.

公开(公告)号：WO2021127637A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/066400

申请日：2020-12-21

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： KENNEDY-DARLING, Julia ,  MILLER, Peter J.

IPC: C12Q1/682 ,  C12Q1/6841

Abstract: Methods for RNA detection in biological samples include (a) contacting a biological sample with a first composition featuring multiple different types of unlabeled oligonucleotide probes that hybridize to RNA species in the sample; (b) contacting the biological sample with a hybridization agent featuring a chaotropic compound; (c) contacting the biological sample with a second composition that includes multiple different types of labeled oligonucleotide probes, where each of the different types of labeled oligonucleotide probes selectively hybridizes to one of the different types of unlabeled oligonucleotide probes; (d) obtaining at least one image of the biological sample with the multiple different types of labeled oligonucleotide probes bound to the sample; and (e) identifying spatial locations of the RNA species in the sample based on components of the at least one image that correspond to the different types of labeled oligonucleotide probes, where the biological sample is contacted with the second composition under isothermal conditions.

公开(公告)号：WO2020163397A2

公开(公告)日：2020-08-13

申请号：PCT/US2020/016667

申请日：2020-02-04

Applicant: AKOYA BIOSCIENCES, INC. ,  DAKSHINAMOORTHY, Gajalakshmi

Inventor： DAKSHINAMOORTHY, Gajalakshmi ,  MILLER, Peter ,  KENNEDY-DARLING, Julia ,  ZHENG, Yi ,  HOYT, Clifford C.

Abstract: The disclosure features methods that include: contacting a biological sample having a first target analyte with a first agent, where the first agent includes a first binding species that specifically binds to the first target analyte, and a first oligonucleotide conjugated to the binding species; contacting the biological sample with a second agent, where the second agent includes a first reactive species and a second oligonucleotide conjugated to the first reactive species, to hybridize at least a portion of the second oligonucleotide to at least a portion of the first oligonucleotide; and contacting the biological sample with a first labeling species, where the first labeling species reacts with the first reactive species to deposit the first labeling species or a derivative thereof in the biological sample.

公开(公告)号：WO2020146552A1

公开(公告)日：2020-07-16

申请号：PCT/US2020/012813

申请日：2020-01-08

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： KENNEDY-DARLING, Julia ,  KIM, Joseph ,  DAKSHINAMOORTHY, Gajalakshmi ,  MCKELLIGON, Brian

IPC: G01N21/64 ,  G01N21/62 ,  G01N33/58 ,  C12Q1/68

Abstract: Provided herein are methods, kits, and compositions for the detection of an element of a biological sample using an antibody conjugated to a first oligonucleotide, which connects to a labelled oligonucleotide for detection via an oligonucleotide partially complementary to the first oligonucleotide and partially complementary to the second oligonucleotide.