公开(公告)号：WO2023014825A1

公开(公告)日：2023-02-09

申请号：PCT/US2022/039323

申请日：2022-08-03

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： MILLER, Peter, J. ,  KENNEDY-DARLING, Julia

IPC: C12Q1/6841 ,  C12Q1/682

Abstract: Methods for labeling biological samples include (a) contacting a biological sample featuring a target RNA with a probe, where the probe includes a capture moiety that specifically binds to the target RNA, and a plurality of reporter moieties, and (b) for each reporter moiety of the plurality of reporter moieties: contacting the reporter moiety with a catalytic agent that includes an oligonucleotide that specifically binds to the reporter moiety, and a reactive species linked to the oligonucleotide, and contacting the biological sample with a labeling agent that reacts with the reactive species to deposit the labeling agent or a derivative thereof in the sample in proximity to the target RNA.