公开(公告)号：WO1993002198A1

公开(公告)日：1993-02-04

申请号：PCT/FI1991000225

申请日：1991-07-23

Applicant: TECHNICAL RESEARCH CENTRE OF FINLAND ,  TEERI, Tuula, T. ,  TAKKINEN, Kristiina ,  LAUKKANEN, Marja-Leena ,  ALFTHAN, Kaija ,  SIZMANN, Dorothea ,  KNOWLES, Jonathan, K., C.

Inventor： TECHNICAL RESEARCH CENTRE OF FINLAND

IPC: C12N15/62

CPC classification number: C07K16/00

Abstract: A method for producing a single chain fusion protein, comprises the steps of: (a) constructing an rDNA molecule coding for a secretable single chain fusion protein having a plurality of functional proteins or protein domains joined by spacer peptides, wherein each such spacer peptide comprises a flexible hinge region of a naturally secreted multidomain protein or an analogue thereof, each adjacent pair of functional proteins or protein domains is joined by a spacer peptide which is not naturally continuous with either functional protein or protein domain to which it is joined, and at least two of the functional proteins or protein domains each independently exhibit physicochemical or biological activity or interact to form an assembly that exhibits physicochemical or biological activity; (b) inserting the rDNA molecule into an expression vector capable of transforming a host organism; (c) transforming a host organism with the resultant vector, and isolating transformants; and (d) culturing the transformants and recovering thesingle chain fusion protein.

Abstract translation: 一种制备单链融合蛋白的方法，包括以下步骤：（a）构建编码具有通过间隔肽连接的多个功能蛋白或蛋白结构域的可分离单链融合蛋白的rDNA分子，其中每个这样的间隔肽包含 天然分泌的多结构域蛋白质或其类似物的柔性铰链区域，每个相邻的功能蛋白质或蛋白质区域对通过间隔肽连接，所述间隔子肽与其所连接的功能性蛋白质或蛋白质结构域不是天然连续的，并且在 功能性蛋白质或蛋白质结构域中至少有两个独立地表现出物理化学或生物活性或相互作用以形成具有物理化学或生物活性的组合物; （b）将rDNA分子插入能够转化宿主生物的表达载体中; （c）用所得载体转化宿主生物体，并分离转化体; （d）培养转化体并回收单链融合蛋白。

公开(公告)号：WO2012085348A1

公开(公告)日：2012-06-28

申请号：PCT/FI2011/051142

申请日：2011-12-20

Applicant: SUOMEN PUNAINEN RISTI, VERIPALVELU ,  GLYKOS FINLAND OY ,  SKOTTMAN, Heli ,  HONGISTO, Heidi ,  VAAJASAARI, Hanna ,  PARTANEN, Jukka ,  ALFTHAN, Kaija

Inventor： SKOTTMAN, Heli ,  HONGISTO, Heidi ,  VAAJASAARI, Hanna ,  PARTANEN, Jukka ,  ALFTHAN, Kaija

IPC: C12N5/071 ,  C12N5/0735

CPC classification number: C12N5/0621 ,  C12N2501/59 ,  C12N2506/02

Abstract: The present invention relates to the production of human retinal pigment epithelial cells comprising a step of incubating human pluripotent stem cells with binder molecules binding to terminal N-acetyllactosamine (Galβ1-4Glc NAc) and/or blood group H determinant type 2 (Fucα1-2Galβ1-4Glc NAc). Particularly, the invention relates to a use of lectin ECA in a culture of human pluripotent stem cells in order to support the stem cells to differentiate into retinal pigment epithelial cells.

Abstract translation: 本发明涉及人视网膜色素上皮细胞的制备，其包括将人多能干细胞与结合末端N-乙酰乳糖胺（Galβ1-4GlcNAc）和/或血型H决定簇2（Fuca1-2Galβ1 -4Glc NAc）。 特别地，本发明涉及凝集素ECA在人多能干细胞的培养物中的用途，以便支持干细胞分化成视网膜色素上皮细胞。