公开(公告)号：WO1992011367A2

公开(公告)日：1992-07-09

申请号：PCT/US1991009810

申请日：1991-12-20

Applicant: MICROCARB INC. ,  HSC RESEARCH AND DEVELOPMENT LIMITED PARTNERSHIP

Inventor： MICROCARB INC. ,  HSC RESEARCH AND DEVELOPMENT LIMITED PARTNERSHIP ,  KRIVAN, Howard, C. ,  NILSSON, Bo. ,  LINGWOOD, Clifford, A.

IPC: C12N15/31

CPC classification number: C07K16/12 ,  A61K38/00 ,  A61K39/00 ,  C07K14/195 ,  C07K14/205 ,  C07K14/295 ,  G01N33/56911

Abstract: Host cell lipid molecules (''receptors'') which permit binding of microorganisms are provided. Pharmaceutical compositions are formed from receptors in combination with a pharmaceutically acceptable carrier or diluent. The present invention also provides microbial proteins (''adhesins''), which mediate binding of microorganisms to host cells, and DNA sequences encoding adhesin proteins. Vaccines are formed from an adhesin protein in combination with a pharmaceutically acceptable carrier or diluent.

Abstract translation: 提供允许微生物结合的宿主细胞脂质分子（“受体”）。 药物组合物由与药学上可接受的载体或稀释剂组合的受体形成。 本发明还提供微生物蛋白质（“粘附素”），其介导微生物与宿主细胞的结合，以及编码粘附素蛋白质的DNA序列。 疫苗由粘附素蛋白与药学上可接受的载体或稀释剂组合形成。

公开(公告)号：WO1992011015A1

公开(公告)日：1992-07-09

申请号：PCT/US1991009800

申请日：1991-12-20

Applicant: MICROCARB INC. ,  HSC RESEARCH AND DEVELOPMENT

Inventor： MICROCARB INC. ,  HSC RESEARCH AND DEVELOPMENT ,  KRIVAN, Howard, C. ,  NILSSON, Bo ,  LINGWOOD, Clifford, A.

IPC: A61K31/685

CPC classification number: A61K31/685 ,  A61K31/70 ,  Y02A50/475 ,  A61K2300/00

Abstract: Compounds and compositions for the in vitro and in vivo inhibition of microbial colonization are provided. In particular, isolated host cell phospholipid molecules (''receptors''), and derivatives thereof, which bind microorganisms are disclosed.

Abstract translation: 提供了用于体外和体内抑制微生物定植的化合物和组合物。 特别地，公开了结合微生物的分离的宿主细胞磷脂分子（“受体”）及其衍生物。

公开(公告)号：WO1993002709A1

公开(公告)日：1993-02-18

申请号：PCT/US1991005422

申请日：1991-07-31

Applicant: MICROCARB INC.

Inventor： MICROCARB INC. ,  KRIVAN, Howard, C. ,  BLOMBERG, Arne, Lennart, Ingemar

IPC: A61K47/48

CPC classification number: A61K47/6911 ,  A61K47/6425

Abstract: A variety of conjugates, comprising at least one agent coupled to at least one receptor which binds a microorganism, are provided. Preferred agents include anti-infectives, such as antibiotics and synthetic drugs. Uses of the conjugates include as in vitro inhibitors and as therapeutic agents, e.g., for the treatment of infections due to pathogenic microorganisms.

Abstract translation: 提供了包含与至少一种结合微生物的受体偶联的至少一种试剂的多种缀合物。 优选的试剂包括抗感染剂，例如抗生素和合成药物。 缀合物的用途包括作为体外抑制剂和治疗剂，例如用于治疗由致病微生物引起的感染。

公开(公告)号：WO1993022423A1

公开(公告)日：1993-11-11

申请号：PCT/US1993004053

申请日：1993-04-29

Applicant: MICROCARB INC.

Inventor： MICROCARB INC. ,  KRIVAN, Howard, C.

IPC: C12N01/20

CPC classification number: C12N1/20 ,  C12N1/38 ,  C12N15/01

Abstract: A variety of methods are provided for growing bacterial cells on lipids, acidic lipids, phospholipids, phosphatidylserine, or mucus, egg or milk fractions or subfractions. Pathogenic bacteria are preferred and include bacteria such as Salmonella, Yersinia, Shigella, Campylobacter, Helicobacter, Pseudomonas, Streptococcus, Staphylococcus, E. coli, Haemophilus, Mycobacterium, Proteus, Klebsiella, Neisseria, Branhamella, Bacteroides, Listeria, Enterococci, Vibrio, Yersinia, Bordetella, Clostridium, Treponema, and Mycoplasma. The present invention also discloses methods for the selection of mutant strains which cannot grow in animals and use of such mutants as host cells for expression of cloned DNA molecules. In addition, methods are provided for the isolation of proteins whose expression is induced or enhanced by growth in the presence of phosphatidylserine or compositions containing phosphatidylserine. Such proteins have a number of uses, including as components of vaccines and as diagnostic markers. The present invention also provides methods for preparing bacteria or fractions thereof for use within cellular or acellular vaccines.

Abstract translation: 提供了多种方法用于在脂质，酸性脂质，磷脂，磷脂酰丝氨酸或粘液，蛋或牛奶级分或亚级分上生长细菌细胞。 致病细菌是优选的，并且包括细菌如沙门氏菌，耶尔森氏菌，志贺氏杆菌，弯曲杆菌，幽门螺杆菌，假单胞菌属，链球菌属，葡萄球菌属，大肠杆菌，嗜血杆菌，分枝杆菌，变形杆菌，克雷伯菌，奈瑟氏球菌，布兰汉菌，拟杆菌属，李斯特氏菌，肠球菌，弧菌属，耶尔森氏菌 ，博德氏菌属，梭菌属，密螺旋体和支原体。 本发明还公开了选择不能在动物中生长的突变菌株和使用这种突变体作为宿主细胞表达克隆的DNA分子的方法。 此外，提供了用于分离其表达在磷脂酰丝氨酸或含有磷脂酰丝氨酸的组合物存在下通过生长诱导或增强的蛋白质分离的方法。 这些蛋白质具有多种用途，包括疫苗的组分和诊断标记。 本发明还提供了在细胞或细胞疫苗中使用的细菌或其部分的制备方法。

公开(公告)号：WO1994000149A1

公开(公告)日：1994-01-06

申请号：PCT/US1993006016

申请日：1993-06-22

Applicant: MICROCARB INC. ,  KRIVAN, Howard, C. ,  SAMUELS, James, E. ,  NORBERG, Nils, Thomas

Inventor： MICROCARB INC.

IPC: A61K39/00

CPC classification number: C07H15/04 ,  A61K39/102 ,  A61K2039/6068 ,  A61K2039/627 ,  C07H3/06 ,  C07H15/08 ,  C07H15/18 ,  C07K14/285 ,  C07K2319/00

Abstract: Disclosed herein are immunogenic polysaccharide-H. influenzae adhesin protein conjugates, a purified H. influenzae adhesin protein and related proteins and polypeptides, DNA useful for producing the proteins, synthetic polyribosylribotol phosphate (PRP) oligosaccharides and intermediates useful for their synthesis, and methods of making and using these materials. The conjugates comprise a PRP fragment, preferably a synthetic oligosaccharide, coupled to an H. influenzae adhesin protein. The invention further comprises purified H. influenzae adhesin proteins and novel PRP oligosaccharides. The invention also comprises methods of producing these materials and using them in a vaccine to protect humans and other mammals against H. influenzae infection.

Abstract translation: 本文公开了免疫原性多糖-H。 流感嗜血杆菌粘附素蛋白质缀合物，纯化的流感嗜血杆菌粘附素蛋白质和相关蛋白质和多肽，可用于产生蛋白质的DNA，合成的聚核糖核糖核酸寡聚糖（PRP）寡糖和可用于其合成的中间体，以及制备和使用这些材料的方法。 缀合物包含与流感嗜血杆菌粘附素蛋白偶联的PRP片段，优选合成寡糖。 本发明还包括纯化的流感嗜血杆菌粘附素蛋白和新型PRP寡糖。 本发明还包括生产这些材料并在疫苗中使用它们以保护人类和其他哺乳动物免于流感嗜血杆菌感染的方法。