公开(公告)号：WO2015011172A1

公开(公告)日：2015-01-29

申请号：PCT/EP2014/065778

申请日：2014-07-23

Applicant: UNIVERSITÄT BIELEFELD

Inventor： NOLL, Thomas ,  KRÄMER, Oliver ,  KLAUSING, Sandra

IPC: C12N15/113 ,  C12N5/071 ,  C12N5/16

CPC classification number: C12N15/113 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/16 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/00 ,  C12N2501/105 ,  C12N2501/59 ,  C12N2510/02

Abstract: This invention relates to methods for the recombinant expression of a protein of interest in a mammalian host cell, use of an sh RNA or an si RNA directed against the Galectin-1 gene for increasing the expression of a protein of interest in a mammalian host cell and kits comprising said shRNA or an si RNA and a CHO cell.

Abstract translation: 本发明涉及用于在哺乳动物宿主细胞中重组表达目标蛋白的方法，使用针对Galectin-1基因的sh RNA或siRNA来增加哺乳动物宿主细胞中目标蛋白的表达 以及包含所述shRNA或siRNA和CHO细胞的试剂盒。