公开(公告)号：WO2022109196A1

公开(公告)日：2022-05-27

申请号：PCT/US2021/059982

申请日：2021-11-18

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： ZHENG, Yi ,  KENNEDY-DARLING, Julia ,  MILLER, Peter J.

IPC: C12Q1/6804 ,  G01N33/533

Abstract: Methods include (a) contacting a biological sample with a composition featuring a plurality of different types of probes, where each type of probe of the plurality of different types of probes includes a detection moiety that selectively binds to a different type of protein target in the sample, a nanobody bound to the detection moiety, and an oligonucleotide linked to the nanobody and featuring an oligonucleotide sequence, where the oligonucleotide sequence of each type of probe is different from the oligonucleotide sequences of each of the other types of probes of the plurality of probes; and (b) contacting the sample with a set of one or more different types of optical labels, where each different type of optical label of the set of optical labels includes an oligonucleotide that selectively hybridizes to only one type of probe. Nanobodies can be labelled accurately with fluorescent molecules, thereby allowing a more accurate imaging of the targets.

公开(公告)号：WO2020252488A2

公开(公告)日：2020-12-17

申请号：PCT/US2020/037821

申请日：2020-06-15

Applicant: AKOYA BIOSCIENCES, INC. ,  DAKSHINAMOORTHY, Gajalakshmi

Inventor： DAKSHINAMOORTHY, Gajalakshmi ,  KENNEDY-DARLING, Julia ,  MILLER, Peter J. ,  HARVEY, Peter

IPC: G01N33/53 ,  G01N33/552

Abstract: An apparatus attaches to a microscope slide to form an enclosed fluidic chamber with input and output ports, where a tissue or cell sample on the slide can be processed using techniques such as immunohistochemical staining. Flow of reagents within the chamber can be laminar and highly uniform across the sample surface to achieve staining that is free of gradients. The sample can be imaged prior to, during, or after any given processing step. Methods for staining and imaging a sample can be implemented through one or more rounds of labeling, label removal or erasure and imaging. Samples can be imaged many times to achieve very high multiplexing levels. Samples can be placed on an imaging apparatus at various times and removed from the imaging apparatus for certain steps.

公开(公告)号：WO2020082087A1

公开(公告)日：2020-04-23

申请号：PCT/US2019/057275

申请日：2019-10-21

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： DANIEL, Steven

IPC: C12Q1/6841 ,  C12Q1/6869

Abstract: Methods for identifying co-occurrence of nucleic acid segments in a nucleic acid sample from a specimen including obtaining a nucleic acid sample from a specimen, determining sequences of first and second nucleic acid segments in nucleic acid fragments of the sample to generate a first and second sets of sequences, generating a first and second sets of probes from the first and second sets of sequences, exposing a detection sample to a member of the first set of probes and a member of the second set of probes, performing a hybridization analysis to determine whether the members of the first and second sets of probes hybridize to the detection sample, and determining whether the first and second nucleic acid segments co-occur in a common cell of the specimen.

公开(公告)号：WO2019213618A1

公开(公告)日：2019-11-07

申请号：PCT/US2019/030735

申请日：2019-05-03

Applicant: AKOYA BIOSCIENCES, INC.

Inventor： MILLER, Peter J. ,  JOHNSON, Kent S. ,  COLTHARP, Carla

IPC: G01N21/64 ,  G01J3/44

Abstract: The disclosure features methods that include exposing a biological sample to illumination light and measuring light emission from the sample to obtain N sample images (104), where each sample image corresponds to a different combination of a wavelength band of the illumination light and one or more wavelength bands of the light emission, where the one or more wavelength bands of the light emission define a wavelength range, and where N > 1, and exposing the sample to illumination light in a background excitation band and measuring light emission from the sample in a background spectral band to obtain a background image of the sample (106), where the background spectral band corresponds to a wavelength within the wavelength range. From the background image, an autofluorescence image is obtained.