ANTIFUNGAL PROTEINS, DNA CODING THEREFOR, AND HOSTS INCORPORATING SAME
    11.
    发明申请
    ANTIFUNGAL PROTEINS, DNA CODING THEREFOR, AND HOSTS INCORPORATING SAME 审中-公开
    抗真菌蛋白,其编码的DNA和与其相同的宿主

    公开(公告)号:WO1995021929A1

    公开(公告)日:1995-08-17

    申请号:PCT/EP1995000488

    申请日:1995-02-09

    IPC分类号: C12N15/82

    摘要: The present invention provides an isolated protein obtainable from a plant source which has anti-Phytophthora activity and a molecular weight of about 60 +/- 5 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterized in that it comprises an open reading frame which is capable of encoding a protein as represented by amino acids 1 to 540 of SEQ ID NO: 6, or the precursor of said protein, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also methods are provided for combatting fungi, especially Phytophthora infestans, using a protein or a host cell capable of producing the protein.

    摘要翻译: 本发明提供了可从植物来源获得的分离蛋白质,其通过SDS PAGE-电泳判断具有抗疫病毒活性和约60 +/- 5kDa的分子量,分离的DNA序列包含能够编码的开放阅读框 根据本发明的蛋白质,其特征在于其包含能够编码由SEQ ID NO:6的氨基酸1至540表示的蛋白质或所述蛋白质的前体的蛋白质的开放阅读框,以及能够 在严格条件下与其杂交。 本发明还包括含有能够编码本发明的蛋白质的嵌合DNA的植物,并且其中所述蛋白质被表达。 还提供了使用能够产生蛋白质的蛋白质或宿主细胞来对抗真菌,特别是致病疫霉的方法。