公开(公告)号：WO2020249670A1

公开(公告)日：2020-12-17

申请号：PCT/EP2020/066198

申请日：2020-06-11

Applicant: UNIBIO A/S

Inventor： NANDY, Subir, Kumar ,  PETERSEN, Leander

IPC: C12N1/30 ,  C12M1/04 ,  C12M1/34

Abstract: The present invention relates to a fermentation process for the fermentation of at least one microorganism, wherein the fermentation process comprises the steps of (a) allowing a fermentation broth comprising the at least one microorganism to flow in the fermentation reactor; (b) supplying a carbon-substrate to the fermentation reactor allowing the gaseous carbon-substrate to be dissolved, or partly dissolved, in the fermentation broth; (c) supplying a nitrogen-substrate to the fermentation reactor allowing the gaseous nitrogen-substrate to be dissolved, or partly dissolved, in the fermentation broth; and (d) maintaining a nitrate concentration of the fermentation broth below 0.035 g/l, and/or maintaining a nitrate concentration of the fermentation broth below 0.01 g nitrate/g biomass; wherein the at least one methanotrophic organism comprises at least one methanotrophic microorganism.