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公开(公告)号：WO2018158319A1
公开(公告)日：2018-09-07
申请号：PCT/EP2018/054944
申请日：2018-02-28
Applicant: UNIBIO A/S
Inventor： HIDAYAT, Budi, Juliman , CHRISTENSEN, Ib , PEDERSEN, Patrick, Nicklas , NANDY, Subir, Kumar , BUSCH-LARSEN, Henrik
Abstract: The present invention relates to a fermentation reactor comprising a loop-part having a circulation pump, a degassing-tank, and a fermentation liquid outlet for withdrawing fermentation liquid from the fermentation reactor, said degassing-tank comprises: (i) a first outlet connecting the degassing-tank to a first leg of the loop-part and allowing fermentation liquid present in the degassing-tank to flow into the loop-part; (ii) a first inlet connecting the degassing-tank to a second leg of the loop-part, allowing fermentation liquid present in the loop-part to flow into the degassing-tank; and (iii) a vent tube for discharging effluent gasses from the degassing tank, such as CO2; wherein one or more of the following criteria applies; (a) (a) wherein the first outlet and/or the first leg of the loop-part is provided with an anti-vortex device to avoid flooding of the circulation pump; (b) wherein a ratio between a volume (internal volume determined as m 3 ) of the degassing-tank relative to a volume (internal volume determined as m 3 ) of the loop-part is in the range of 0.25: 1 to 20: 1; (c) wherein a numerical ratio between a volume (internal volume, determined as m 3 ) of the degassing-tank relative to a cross section area of the first leg and/or the cross section area (determined as m 2 ) of second leg is in the range of 10: 1 to 250: 1; (d) wherein a cross section area of the first leg (determined as m 2 ) is 10% or more larger than a cross section area of the second leg (determined as m 2 ), (e) wherein a cross section area of the second leg (determined as m 2 ) is 10% or more larger than a cross section area of the first leg (determined as m 2 ), or (f) any combination of criteria (a) to (e).
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公开(公告)号：WO2020245197A1
公开(公告)日：2020-12-10
申请号：PCT/EP2020/065347
申请日：2020-06-03
Applicant: UNIBIO A/S
Inventor： NANDY, Subir, Kumar , PETERSEN, Leander , CHRISTENSEN, Ib
Abstract: The present invention relates to a process for cultivating one or more microorganisms capable of metabolising methane, the process comprises the steps of: (i) adding a fermentation medium to a fermentation reactor; (ii) adding the one or more microorganism to a fermentation reactor, providing an inoculated fermentation medium, wherein the one or more microorganism does not include a recombinant microorganism; (iii) adding a C1-C5 carbon source, e.g. methane, to the fermentation reactor and/or the inoculated fermentation medium during the fermentation of the one or more microorganisms; and (iv) optionally, adding oxygen to the fermentation reactor and/or the inoculated fermentation medium during the fermentation of the one or more microorganisms, wherein the oxygen is added to the fermentation reactor and/or the inoculated fermentation medium to provide a content of undissolved oxygen in the fermentation reactor and/or a content of gaseous oxygen in an exhaust gas is at most 5% (vol/vol).
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公开(公告)号：WO2019063809A1
公开(公告)日：2019-04-04
申请号：PCT/EP2018/076502
申请日：2018-09-28
Applicant: UNIBIO A/S
Inventor： CHRISTENSEN, Ib , PETERSEN, Leander , DREJER, André, Kofoed , JØRGENSEN, John, Bagterp , JØRGENSEN, Sten, Bay , KNUDSEN, Jørgen, K. H.
Abstract: The present invention relates to a system and a method for adjusting and/or optimize a fermentation process performed in at least one U-loop fermenter, the system comprising: at least one U-loop fermenter being operatively connected to a computer; the computer comprising a processor and being operatively connected to a database; at least one 5 analysis apparatus for repetitively providing a test value of at least one parameter of one U-loop fermenter; a data interface for repetitively entering the test value of the at least one parameter in the database; the database comprising at least one control value; wherein the database is adapted to store multiple database entries representing the test value of the at least one parameter at various points in time and adapted to store multiple 10 database entries representing the control value at various points in time, wherein the processor is programmed to: perform at least one mathematical analysis of the test value providing a calculated test value, and/or the control value providing at least one calculated control value; and selecting, on the basis of the calculated test value and/or on the calculated control value, the adjustment to be introduced into at least one other U-loop fermenter to benefit from the change made in the one U-loop fermenter.
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公开(公告)号：WO2018115042A1
公开(公告)日：2018-06-28
申请号：PCT/EP2017/083683
申请日：2017-12-20
Applicant: UNIBIO A/S
Inventor： NTOKOU, Eleni , NANDY, Subir, Kumar
Abstract: The present invention relates to a method for providing a SCP product from a biomass material wherein said SCP product comprising a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids in the biomass material, the method comprising the steps of: (i) providing the biomass material; (ii) subjecting the biomass material to a process of cell disruption, providing a disrupted biomass material; (iii) applying the disrupted biomass material to a first separation process resulting in a first retentate comprising proteins and/or cell debris, and a first permeate comprising nucleic acids; (iv) subjecting the first permeate to a second treatment separating the nucleic acids from vitamins, minerals and/or amino acids; (v) optionally, combining the first retentate obtained in step (iii) with the vitamins, minerals and/or amino acids obtained in step (iv), providing the SCP product comprising a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids.
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公开(公告)号：WO2020244961A1
公开(公告)日：2020-12-10
申请号：PCT/EP2020/064510
申请日：2020-05-26
Applicant: UNIBIO A/S
Inventor： JULEGAARD, Ina, Karlshøj , JENSEN, Michael
IPC: A23K10/16 , A23K20/142 , A23K20/147 , A23K50/30 , A23K50/60 , A23K20/20
Abstract: The present invention relates to a pig feed comprising a single cell protein (SCP) or a fraction hereof.
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Patent Agency Ranking
公开(公告)号：WO2020152343A1
公开(公告)日：2020-07-30
申请号：PCT/EP2020/051787
申请日：2020-01-24
Applicant: UNIBIO A/S
Inventor： CHRISTENSEN, Ib
IPC: C12M1/00
Abstract: The present invention relates to a fermentation reactor comprising a loop-part and a top tank, said loop-part comprising a downflow part, connected to an upflow part via a U-part, wherein the top tank comprises: (i) a first outlet connecting the top tank to the downflow part of the loop-part and allowing a fermentation liquid present in the top tank to flow from the top tank into the loop-part; (ii) a first inlet connecting the top tank to the upflow part of the loop-part, allowing fermentation liquid present in the loop-part to flow from the loop part into the top tank; and (iii) a vent tube for discharging effluent gasses from the top tank; wherein the top tank further comprises a visual inspection means.
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7.发明申请
公开(公告)号：WO2018158322A1
公开(公告)日：2018-09-07
申请号：PCT/EP2018/054947
申请日：2018-02-28
Applicant: UNIBIO A/S
Inventor： NANDY, Subir, Kumar , CHRISTENSEN, Ib , HIDAYAT, Budi, Juliman
IPC: C12N1/20
Abstract: The present invention relates to a method for providing a fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism, the method comprises the step of: (i) providing a main growth solution; (ii) optionally sterilizing the main growth solution provided in step (i); (iii) providing a trace metal solution; and (iv) mixing the main growth solution (as provided in step (i) or (ii)) with the trace metal solution provided in step (iii) and providing the fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism.
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公开(公告)号：WO2017080987A2
公开(公告)日：2017-05-18
申请号：PCT/EP2016/076950
申请日：2016-11-08
Applicant: UNIBIO A/S
Inventor： NANDY, Subir, Kumar
Abstract: The present invention relates to a method for improving biomass production and/or growth rate of a microorganism in a fermentation process, said method comprises the steps of: (i) providing one or more microorganism; (ii) providing a fermentation substrate suitable for fermenting the one or more microorganism; (iii) mixing the one or more microorganism and the fermentation substrate providing a fermentation broth; (iv) adding the fermentation broth to a fermentation tank; (v) injecting at least one gaseous substrate into the fermentation broth; (vi) running the fermentation process for a fermentation period of at least 1 hour; wherein the at least one gaseous substrate comprises one or more greenhouse gases, such as carbon dioxide (CO 2 ).
Abstract translation: 本发明涉及用于改善发酵过程中微生物的生物量产生和/或生长速率的方法，所述方法包括以下步骤：（i）提供一种或多种微生物; （ii）提供适用于发酵所述一种或多种微生物的发酵底物; （iii）将所述一种或多种微生物与提供发酵液的所述发酵基质混合; （iv）将发酵液加入发酵罐中; （v）将至少一种气态底物注入发酵液中; （vi）将发酵过程运行至少1小时的发酵时间; 其中所述至少一种气态底物包含一种或多种温室气体，例如二氧化碳（CO 2）。
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公开(公告)号：WO2022008478A2
公开(公告)日：2022-01-13
申请号：PCT/EP2021/068582
申请日：2021-07-06
Applicant: UNIBIO A/S
Inventor： NANDY, Subir, Kumar , CHRISTENSEN, Ib
Abstract: The present invention relates to a process for providing a first reaction product by a first fermentation process conducted in a first Loop reactor, the method comprising the steps of: (i) adding an inoculum comprising one or more methanogenic microorganism to the first Loop reactor providing a first inoculated fermentation medium; (ii) adding a gaseous hydrogen (H2) to the first inoculated fermentation medium; (iii) adding a first carbon source to the first inoculated fermentation medium; (iv) allowing the first fermentation medium to ferment, providing the first reaction product; and (v) isolating the first reaction product provided in step (iv).
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公开(公告)号：WO2021116366A1
公开(公告)日：2021-06-17
申请号：PCT/EP2020/085681
申请日：2020-12-11
Applicant: UNIBIO A/S
Inventor： CHRISTENSEN, Ib , ELLESKOV, Michael
Abstract: The present invention relates to a static mixer or a flow reactor comprising a static mixer for mixing streams of fluid transported through or circulated in a flow reactor, and in particular a static mixer according to the invention may improve gas/mass transfer by reducing the size of gas bubbles. A flow reactor according to the invention may have any cross-section, e.g. circular, and one or more mixing units may be inserted in the flow reactor. More particularly, the invention relates to a static mixer element configured to reduce the size of gas bubbles in a liquid for installation in a flow channel which static mixer element comprises a mixer unit (1) extending over a cross-section area of the flow channel which mixer unit (1) comprises a plurality of through openings (3), the static mixer element comprises or is constituted of at least two mixer units (1), a first or previous mixer unit (1) and a second and adjacent mixer unit (1), which first and second mixer units are displaced relative to each other in the direction of flow, in such a way that a through opening (3) in the first or previous mixer unit (1) at least partly faces a surface of the second and adjacent mixer unit (1) thereby forcing the fluid flow to change direction, wherein adjacent mixer units (1) have substantially the same height or thickness h, and are substantially parallel having a distance d between them, which distance d may be approximately 0 as two adjacent mixer units (1) may be in contact with each other at one or more points, or the distance d may be larger than 0 and may be at least 0.5 the thickness of a mixer unit (1) i.e. 0.5xh, or the distance d may be at least the thickness of a mixer unit i.e. 1xh, or the distance d may be at maximum 10 times the thickness of a mixer unit (1) i.e. 10xh, or at maximum 5 times the thickness of a mixer unit i.e. 5xh, or at maximum 3 times the thickness of a mixer unit i.e. 3xh.
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