公开(公告)号：WO2023009622A1

公开(公告)日：2023-02-02

申请号：PCT/US2022/038520

申请日：2022-07-27

申请人： ATSENA THERAPEUTICS, INC.

发明人： KOZLOWSKI, Christopher

IPC分类号： C12Q1/68 ,  A61K35/76 ,  C07H21/04

摘要： Disclosed herein is a nucleic acid stuffer sequence comprising one or more of the following features: no CPG island and no more than four contiguous nucleobases of the same identity; wherein the nucleic acid stuffer sequences does not include an open reading frame greater than 20 amino acids in length. In some embodiments, the nucleic acid stuffer sequence comprises between about 40% and about 50% GC content. In some embodiments, the nucleic acid stuffer sequence does not comprise a restriction enzyme cleavage site. In some embodiments, the nucleic acid stuffer sequence has a length of about 100 nucleobases to about 5000 nucleobases.