公开(公告)号：WO1990012091A1

公开(公告)日：1990-10-18

申请号：PCT/DK1990000090

申请日：1990-04-09

Applicant: CANCERFORSKNINGSFONDET AF 1989 ,  DANO, Keld ,  BLASI, Francesco ,  ROLDAN, Ann, Louring ,  CUBELLIS, Maria, Vittoria ,  MASUCCI, Maria, Teresa ,  APPELLA, Ettore ,  SCHLEUNING, W., D. ,  BEHRENDT, Niels ,  RONNE, Ebbe ,  KRISTENSEN, PETER ,  PÖLLÄNEN, Jari ,  SALONEN, Eeva-Marjatta ,  STEPHENS, Ross, W. ,  TAPIOVAARA, Hannele ,  VAHERI, Antii ,  MOLLER, Lisbeth, Birk ,  ELLIS, Vincent ,  LUND, Leif, Roge ,  PLOUG, Michael ,  PYKE, Charles ,  PATTHY, László

Inventor： CANCERFORSKNINGSFONDET AF 1989

IPC: C12N09/72

CPC classification number: C12N9/6462 ,  A61K38/00 ,  A61K38/1808 ,  A61K38/49 ,  A61K39/3955 ,  C07K14/705 ,  C07K16/28 ,  C07K16/2896 ,  C07K2317/76 ,  C07K2317/77 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Y304/21073 ,  G01N33/57446 ,  G01N33/86 ,  G01N2333/9723 ,  A61K2300/00

Abstract: Pure human cellular receptor for urokinase plasminogen activator (u-PAR) and forms thereof, including a truncated water-soluble form produced by cleaving or removing a glycosyl-phosphatidylinositol anchor, are provided, as is cDNA for u-PAR. Cell surface plasminogen activation, believed to play an important role in tissue remodelling, particularly during cancer invasion, is inhibited by antibodies against u-PAR and by inactivated analogues of u-PA. In situ hybdridization showed presence of u-PAR mRNA in the invasion front of colon carcinomas, proving the diagnostic utility of the cDNA. Water-soluble u-PAR is useful for inhibition of u-PA catalyzed plasminogen activation in solution.

Abstract translation: 提供了用于尿激酶纤溶酶原激活物（u-PAR）的纯人细胞受体及其形式，包括通过切割或除去糖基 - 磷脂酰肌醇锚点产生的截短的水溶性形式，如u-PAR的cDNA。 被认为在组织重塑中起重要作用的细胞表面纤溶酶原激活，特别是在癌症侵袭期间，被u-PAR抗体和u-PA的灭活类似物所抑制。 原位杂交显示u-PAR mRNA在结肠癌侵袭前的存在，证明了cDNA的诊断效用。 水溶性u-PAR可用于抑制溶液中u-PA催化的纤溶酶原激活。