公开(公告)号：WO2004053156A1

公开(公告)日：2004-06-24

申请号：PCT/EP2003/013905

申请日：2003-12-08

Applicant: INNOGENETICS N.V. ,  ROCHE DIAGNOSTICS GMBH ,  DE HENAU, Hilde ,  VAN CROMBRUGGEN, Joachim ,  JANNES, Geert ,  HABERHAUSEN, Gerd ,  EMRICH, Thomas

Inventor： DE HENAU, Hilde ,  VAN CROMBRUGGEN, Joachim ,  JANNES, Geert ,  HABERHAUSEN, Gerd ,  EMRICH, Thomas

IPC: C12Q1/68

CPC classification number: C12Q1/689

Abstract: The present invention relates to a method for the specific detection and/or identification of Enterococcus species, in particular Enterococcus faecalis and/or Enterococcus faecium, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Enterococcus species, in particular of Enterococcus faecalis and/or Enterococcus faecium, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Enterococcus species in a sample.

Abstract translation: 本发明涉及使用源自ITS（内转录间隔区）的新核酸序列来特异性检测和/或鉴定肠球菌属物种，特别是粪肠球菌和/或屎肠球菌的方法。本发明还涉及 对来自ITS区域的所述新的核酸序列，16S和23S核糖核糖核酸（rRNA）或rRNA基因之间，用于特异性检测和/或鉴定肠球菌属物种，特别是粪肠球菌和/或 生物样品中的屎肠球菌。还涉及用于在样品中扩增肠球菌属物种的所述间隔区的核酸引物。